Effect of Recombinant Human Interferon-αA/D on in Vivo Murine Tumor Cell Growth
Effect of Recombinant Human Interferon-αA/D on in Vivo Murine Tumor Cell Growth
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重组人干扰素-αA/D对体内小鼠肿瘤细胞生长的影响
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
S. Muramatsu
中科院分区：
文献类型：
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作者：
K. Uno;S. Shimizu;K. Inaba;M. Kitaura;K. Nakahira;Takuma Kato;Y. Yamaguchi;S. Muramatsu
我们研究了人重组干扰素-αA/D (A/D-IFN)（已知可延迟小鼠肿瘤细胞的生长）对小鼠腹腔内小鼠 Meth A 纤维肉瘤 S1 和 R1 亚系细胞生长的影响。 S1 细胞的体外生长对 A/D-IFN 的直接作用敏感，而 R1 细胞的体外生长对 A/D-IFN 的直接作用具有抵抗力，就像最初用于分离这些亚系的鼠天然 IFN-α/β 一样。然而，在体内，A/D-IFN的施用不仅抑制了S1细胞的生长，而且还抑制了R1细胞的生长，并且荷瘤小鼠的存活时间延长了。尽管A/D-IFN在体内对S1细胞有直接作用，但R1细胞仅对通过宿主细胞的间接作用敏感。从经 A/D-IFN 处理的携带腹水 R1 细胞的小鼠腹腔中收获的巨噬细胞 (Mo) 在抑制 R1 细胞的体外生长方面非常有效；来自不携带 R1 的 A/D-IFN 治疗小鼠的效果较差。体外实验结果表明，Mo很可能是由A/D-IFN和荷瘤小鼠淋巴细胞产生的Mo激活因子的协同作用激活的。
We investigated the effect of human recombinant interferon-αA/D (A/D-IFN), which is known to delay the growth of murine tumor cells, on the growth of S1 and R1 subline cells of murine Meth A fibrosarcoma in the peritoneal cavity of mice. In vitro growth of S1 cells was sensitive to, and that of R1 cells was resistant to, the direct effect of A/D-IFN, as with murine natural IFN-α/β, which was used originally to isolate these sublines. In vivo , however, the growth of not only S1 cells but also R1 cells was suppressed by the administration of A/D-IFN, and the survival time of tumor-bearing mice was prolonged. Although A/D-IFN had a direct effect on S1 cells in vivo , R1 cells were susceptible only to the indirect effect via the host cells. Macrophages (Mo) harvested from the peritoneal cavity of A/D-IFN-treated mice bearing ascitic R1 cells were very effective in suppressing the in vitro growth of R1 cells; those from non-R1-bearing A/D-IFN-treated mice were less effective. The results of in vitro experiments indicate that Mo are very probably activated by the synergism of A/D-IFN and Mo-activating factor(s) produced by lymphoid cells in tumor-bearing mice.