3D-Printed Soft Magnetoelectric Microswimmers for Delivery and Differentiation of Neuron-Like Cells
3D-Printed Soft Magnetoelectric Microswimmers for Delivery and Differentiation of Neuron-Like Cells
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3D打印软磁电微型游泳器用于神经元样细胞的输送和分化
DOI：
10.1002/adfm.201910323
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发表时间：
2020-03-01
影响因子：
19
通讯作者：
Pane, Salvador
中科院分区：
文献类型：
--
作者：
Dong, Mei;Wang, Xiaopu;Pane, Salvador
神经退行性疾病通常会导致不可逆转的神经元损伤和神经元死亡。细胞疗法有望成为治疗这些疾病的一种潜在疗法。然而，这些细胞的治疗性靶向输送和原位提供合适的微环境以使其分化为功能神经元网络仍然具有挑战性。介绍了一种高度集成的多功能软螺旋微泳器，其特点是靶向递送神经细胞，按需定位无线神经元电刺激，以及产后酶降解。利用光固化明胶-甲基丙烯酰基(GelMA)水凝胶的双光子光刻技术制作了微泳者的螺旋软体。然后在螺旋体上浸入显示磁电特性(MENPs)的复合多铁纳米颗粒。柔软的GelMA水凝胶底盘支持细胞生长，并被细胞分泌的酶降解，而MENPs允许生物活性底盘的磁性运输，并充当神经元样细胞的磁性电刺激器。这些材料的独特组合使这些微泳者具有高度集成的设备，可以满足其未来转化为临床应用的几项要求，如货物输送、细胞刺激和生物降解性。作者设想，这些设备将为创伤性损伤和中枢神经系统疾病的靶向细胞治疗开辟新的途径。
Neurodegenerative diseases generally result in irreversible neuronal damage and neuronal death. Cell therapy shows promise as a potential treatment for these diseases. However, the therapeutic targeted delivery of these cells and the in situ provision of a suitable microenvironment for their differentiation into functional neuronal networks remain challenging. A highly integrated multifunctional soft helical microswimmer featuring targeted neuronal cell delivery, on-demand localized wireless neuronal electrostimulation, and post-delivery enzymatic degradation is introduced. The helical soft body of the microswimmer is fabricated by two-photon lithography of the photocurable gelatin-methacryloyl (GelMA)-based hydrogel. The helical body is then impregnated with composite multiferroic nanoparticles displaying magnetoelectric features (MENPs). While the soft GelMA hydrogel chassis supports the cell growth, and is degraded by enzymes secreted by cells, the MENPs allow for the magnetic transportation of the bioactive chassis, and act as magnetically mediated electrostimulators of neuron-like cells. The unique combination of the materials makes these microswimmers highly integrated devices that fulfill several requirements for their future translation to clinical applications, such as cargo delivery, cell stimulation, and biodegradability. The authors envision that these devices will inspire new avenues for targeted cell therapies for traumatic injuries and diseases in the central nervous system.