Sycp1 Is Not Required for Subtelomeric DNA Double-Strand Breaks but Is Required for Homologous Alignment in Zebrafish Spermatocytes.
Sycp1 Is Not Required for Subtelomeric DNA Double-Strand Breaks but Is Required for Homologous Alignment in Zebrafish Spermatocytes.
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DOI：
10.3389/fcell.2021.664377
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发表时间：
2021
影响因子：
5.5
通讯作者：
Sakai N
中科院分区：
文献类型：
--
作者：
Imai Y;Saito K;Takemoto K;Velilla F;Kawasaki T;Ishiguro KI;Sakai N
在减数分裂前期I，同源染色体通过联会复合体结合在一起，联会复合体中两个轴向元件通过横丝和中心元件蛋白连接。在人类和斑马鱼精母细胞中，联会复合体的同源重组和组装主要在端粒附近启动。在小鼠中，突触是不需要的减数分裂双链断裂（DSB）和同源物对齐，但需要DSB修复，然而，这些减数分裂事件中的背景下，端粒偏置的相互作用仍然不清楚。在这项研究中，我们确定了一个过早停止突变的斑马鱼基因编码的横丝蛋白Sycp 1。在sycp 1突变的斑马鱼精母细胞，轴元件形成和配对的同源染色体末端之间的早期到中期的接合线。然而，他们没有突触，他们的协会大多是在偶线期或粗线期晚期丢失。在sycp 1突变型精母细胞中，观察到γ H2 AX信号，Dmc 1/Rad 51和RPA信号主要出现在端粒附近，类似于野生型表型。我们观察到持续本地化的Hormad 1沿着轴sycp 1突变精母细胞，而大多数的Iho 1信号出现和消失的动力学类似的野生型精母细胞。值得注意的是，观察到持续Iho 1灶spo 11突变精母细胞，这表明Iho 1解离轴发生DSB依赖的方式。我们的研究结果表明，Sycp 1是不需要的peritelomeric DSB形成，但在斑马鱼减数分裂的同源物的完全配对是必要的。
In meiotic prophase I, homologous chromosomes are bound together by the synaptonemal complex, in which two axial elements are connected by transverse filaments and central element proteins. In human and zebrafish spermatocytes, homologous recombination and assembly of the synaptonemal complex initiate predominantly near telomeres. In mice, synapsis is not required for meiotic double-strand breaks (DSBs) and homolog alignment but is required for DSB repair; however, the interplay of these meiotic events in the context of peritelomeric bias remains unclear. In this study, we identified a premature stop mutation in the zebrafish gene encoding the transverse filament protein Sycp1. In sycp1 mutant zebrafish spermatocytes, axial elements were formed and paired at chromosome ends between homologs during early to mid-zygonema. However, they did not synapse, and their associations were mostly lost in late zygotene- or pachytene-like stages. In sycp1 mutant spermatocytes, γH2AX signals were observed, and Dmc1/Rad51 and RPA signals appeared predominantly near telomeres, resembling wild-type phenotypes. We observed persistent localization of Hormad1 along the axis in sycp1 mutant spermatocytes, while the majority of Iho1 signals appeared and disappeared with kinetics similar to those in wild-type spermatocytes. Notably, persistent Iho1 foci were observed in spo11 mutant spermatocytes, suggesting that Iho1 dissociation from axes occurs in a DSB-dependent manner. Our results demonstrated that Sycp1 is not required for peritelomeric DSB formation but is necessary for complete pairing of homologs in zebrafish meiosis.
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