NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor
NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor
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DOI：
10.1360/03tb9076
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发表时间：
2003-02-01
影响因子：
--
通讯作者：
Tang, ZC
中科院分区：
文献类型：
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作者：
Hu, XY;Neill, SJ;Tang, ZC
稻瘟病菌特异小种98-186- 1G 1和97-23- 2D 1分别与水稻秀水产生不亲和反应和亲和反应。从M.稻瘟病菌小种98-186- 1G 1（简称1 E）诱导水稻“秀水”悬浮培养的HR反应，包括PAL活性增加，pal、pr 1、chi的转录，细胞死亡和NO的产生。命名为CE的稻瘟病菌小种97-23- 2D 1在诱导这种效应方面效率低得多。NOS酶抑制剂L-NAA和PBITU抑制了1 E诱导的秀水水稻NO的产生。诱导PAL活性和pr 1、pal、chi基因转录增加。L-NAA、PBITU或CPTIO预处理可阻断1 E。用NO供体（SNP）直接处理水稻培养物也诱导了pr 1、pal和chi基因的转录。这些数据表明，NO作为一个信号介导的HR在水稻诱导的IE，并表明NO，与H2 O2，是必要的诱导细胞死亡的IE在水稻悬浮细胞。
Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice "Xiushui". The elicitor from the cell wall of M. grisea race 98-186-1G1, termed 1E, strongly induced the HR response in suspension cultures of rice "Xiushui", including increased PAL activity, transcription of pal, pr1, chi, cell death and the generation of NO. The elicitor prepared from the cell wall of M. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by 1E in "Xiushui" rice. The increased PAL activity and transcription of pr1, pal, chi genes induced by. 1E were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription of pr1, pal and chi genes. These data implicated that NO acted as a signal mediating the HR induced by 1E in rice and showed that NO, in combination with H2O2, is necessary for induction of cell death by IE in rice suspension cells.