Alterations in cellular RNA decapping dynamics affect tomato spotted wilt virus cap snatching and infection in Arabidopsis
Alterations in cellular RNA decapping dynamics affect tomato spotted wilt virus cap snatching and infection in Arabidopsis
复制标题
拟南芥细胞RNA脱帽动力学的变化影响番茄斑萎病毒的帽抢夺和感染
DOI：
10.1111/nph.16049
复制
发表时间：
2019-08-16
期刊：
影响因子：
9.4
通讯作者：
Moffett, Peter
中科院分区：
文献类型：
--
作者：
Ma, Xiaofang;Zhou, Yijun;Moffett, Peter
RNA加工和衰变途径对RNA病毒有重要影响，特别是感染动物的布尼亚病毒，它们利用cap-snatching机制翻译其mrna。然而，它们对植物感染的布尼亚病毒的作用尚未被研究。以拟南芥为研究对象，研究了转录因子decapapping 2 （DCP2）、EXORIBONUCLEASE 4 （XRN4）、ASYMMETRIC LEAVES2 （AS2）和UP-FRAMESHIFT 1 （UPF1）在布雅病毒、番茄斑点枯萎病毒（TSWV）等RNA病毒侵染过程中的作用。感染TSWV后，RNA脱帽能力增强或减弱的突变体对TSWV的易感性分别增加和降低。相比之下，这些突变对使用不同mRNA封顶策略的RNA病毒有相反的影响，或者没有影响。与此一致的是，dcp2突变体中TSWV mRNA的RNA盖帽效率更高。此外，TSWV N蛋白与RNA加工体（PB）组分部分共定位，通过热休克或与其他病毒共感染改变脱帽活性，导致TSWV积累发生相应的变化。目前的研究结果表明，TSWV在植物中的感染取决于其从预定在PBs中脱帽的mrna上夺取帽的能力，并且RNA加工动力学的遗传或环境改变可以影响感染结果。
RNA processing and decay pathways have important impacts on RNA viruses, particularly animal-infecting bunyaviruses, which utilize a cap-snatching mechanism to translate their mRNAs. However, their effects on plant-infecting bunyaviruses have not been investigated. The roles of mRNA degradation and non-sense-mediated decay components, including DECAPPING 2 (DCP2), EXORIBONUCLEASE 4 (XRN4), ASYMMETRIC LEAVES2 (AS2) and UP-FRAMESHIFT 1 (UPF1) were investigated in infection of Arabidopsis thaliana by several RNA viruses, including the bunyavirus, tomato spotted wilt virus (TSWV). TSWV infection on mutants with decreased or increased RNA decapping ability resulted in increased and decreased susceptibility, respectively. By contrast, these mutations had the opposite, or no, effect on RNA viruses that use different mRNA capping strategies. Consistent with this, the RNA capping efficiency of TSWV mRNA was higher in a dcp2 mutant. Furthermore, the TSWV N protein partially colocalized with RNA processing body (PB) components and altering decapping activity by heat shock or coinfection with another virus resulted in corresponding changes in TSWV accumulation. The present results indicate that TSWV infection in plants depends on its ability to snatch caps from mRNAs destined for decapping in PBs and that genetic or environmental alteration of RNA processing dynamics can affect infection outcomes.