Effects of rooting media on root growth and morphology of Brassica rapa seedlings

Effects of rooting media on root growth and morphology of Brassica rapa seedlings
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生根介质对白菜幼苗根系生长和形态的影响

DOI:
10.1080/02571862.2016.1141336
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发表时间:
2016
影响因子:
0.9
通讯作者:
Adu M
Adu M
中科院分区:
--
文献类型:
--
作者:
Adu M

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在目前的根表型研究中使用的生根培养基可以有实质性的影响。在本研究中,三种不同的营养传导纸(黑色建筑纸,锚 以芜菁品种“R500”和“IMB 211”为试材,研究了蓝发芽纸和Kimpakpaper处理和土箱处理对芜菁根系生长和根系构型的影响。幼苗 两个B。在营养传导纸上和土壤填充盒中向芜菁基因型提供营养。纸张和装满泥土的盒子被固定在平板扫描仪上, 定期拍摄和分析根的二维图像。根介质的影响,地上部和根的生物量和拓扑指数(TI)进行了观察。例如, 在建筑纸上更明显。R500和IMB 211在结构纸上分别记录的平均TI为0.82和0.93,表明基材影响人字形结构 Brassica根的图案。同时指出,当使用不同的发芽纸时,对于相同的RSA可以得到不同的结果,结果表明,锚蓝 发芽纸是RSA性状和根生长的表型幼苗中土壤的理想替代物。
Rooting media used in current root phenotyping studies can have substantial effect. In this study, the effects of three different nutrient conducting papers (Black construction paper, Anchor blue germination paper and Kimpakpaper) and soil-filled boxes on root growth and root system architecture (RSA) of Brassica rapa (cultivars 'R500'and 'IMB211') were investigated. Seedlings of the two B. rapa genotypes were supplied with nutrients on the nutrient conducting papers and in the soil-filled boxes. The papers and soil-filled boxes were fixed to flatbed scanners and two-dimensional images of roots were periodically taken and analysed. Root media effects on shoot and root biomass and on topological indices (TI) were observed. For example, root branching was more pronounced on the construction paper. Mean TI of 0.82 and 0.93, recorded for R500 and IMB211, respectively, on the construction paper indicated that substrates affect the herringbone pattern of brassica roots. Whilst it was indicated that different results could be obtained for the same RSA when different germination papers are used, the results showed that Anchor blue germination paper is an ideal proxy for soil in phenotyping seedlings for RSA traits and root growth.
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