Further support for aneuploidy tolerance in wild yeast and effects of dosage compensation on gene copy-number evolution
Further support for aneuploidy tolerance in wild yeast and effects of dosage compensation on gene copy-number evolution
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DOI：
10.7554/elife.14409
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发表时间：
2016-03-07
期刊：
影响因子：
7.7
通讯作者：
Wang, Zhishi
中科院分区：
文献类型：
--
作者：
Gasch, Audrey P.;Hose, James;Wang, Zhishi
在Hose等人之前的工作中，我们进行了一项基因组测序调查，并报道了在野生型沙门氏菌中经常观察到非整倍体。啤酒。我们还分析了与同基因整倍体对照相比天然非整倍体分离物中的转录组丰度，并发现10 - 30%的扩增基因（取决于菌株和受影响的染色体）与基因拷贝数相比显示出低于预期的表达。在Hose等人的文献中，我们认为，该基因组富含一种或多种剂量补偿模式的基因，其中mRNA丰度响应于该基因的较高剂量而降低。托雷斯等人最近的一份手稿驳斥了我们先前的工作。在这里，我们提供了一个回应托雷斯等人，沿着额外的分析和控制来支持我们最初的结论。我们认为，非整倍体是很好的耐受性在野生菌株的S。我们发现，我们研究的酿酒酵母的基因组和基因富集的那些受剂量补偿显示出独特的进化特征。
In our prior work by Hose et al., we performed a genome-sequencing survey and reported that aneuploidy was frequently observed in wild strains of S. cerevisiae. We also profiled transcriptome abundance in naturally aneuploid isolates compared to isogenic euploid controls and found that 10-30% of amplified genes, depending on the strain and affected chromosome, show lower -than -expected expression compared to gene copy number. In Hose et al., we argued that this gene group is enriched for genes subject to one or more modes of dosage compensation, where mRNA abundance is decreased in response to higher dosage of that gene. A recent manuscript by Torres et al. refutes our prior work. Here, we provide a response to Torres et al., along with additional analysis and controls to support our original conclusions. We maintain that aneuploidy is well tolerated in the wild strains of S. cerevisiae that we studied and that the group of genes enriched for those subject to dosage compensation show unique evolutionary signatures.