ROLE OF SEX HORMONES IN HYPERCAPNIA-INDUCED ACTIVATION OF THE LOCUS COERULEUS IN FEMALE AND MALE RATS
ROLE OF SEX HORMONES IN HYPERCAPNIA-INDUCED ACTIVATION OF THE LOCUS COERULEUS IN FEMALE AND MALE RATS
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DOI：
10.1016/j.neuroscience.2015.11.026
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发表时间：
2016-01-28
期刊：
影响因子：
3.3
通讯作者：
Gargaglioni, L. H.
中科院分区：
文献类型：
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作者：
De Carvalho, D.;Marques, D. A.;Gargaglioni, L. H.
蓝斑（LC）被认为是哺乳动物的CO2化学感受器部位。大多数涉及LC在高碳酸血症缓解反应中作用的研究均在男性中进行。由于卵巢类固醇调节LC神经元的活性和女性有不同的呼吸反应，二氧化碳比男性，我们评价了LC去甲肾上腺素能神经元的活性在正常碳酸血症和高碳酸血症的女性和男性大鼠不同的性激素水平。对去卵巢（OVX）、雌二醇（E2）处理的去卵巢（OVX+ E2）大鼠和发情周期中非发情日的雌性大鼠进行了评价。同时，研究了性腺完整、去势（ORX）、睾酮（T）处理的ORX（ORX + T）和E2处理的ORX（ORX+ E2）雄性动物。LC神经元的激活通过c-Fos和酪氨酸羟化酶（TH）的双标记免疫组织化学测定。7%CO2诱导的高碳酸血症使各组LC中c-Fos/TH免疫反应阳性（ir）神经元的数量增加。高碳酸血症诱导的c-Fos表达在发情期雌性和完整雄性大鼠之间没有差异。与OVX大鼠相比，OVX+ E2组大鼠在正常碳酸血症时c-Fos表达减弱，但CO（2）反应性没有改变。此外，在ORX大鼠，无论是T或E2治疗LC去甲肾上腺素能神经元的c-Fos表达的变化。因此，在雌性大鼠中，E2减少LC去甲肾上腺素能神经元的激活，而在雄性中，性激素不影响LC活性。(C)2015年IBRO。由爱思唯尔有限公司发布。保留所有权利。
The locus coeruleus (LC) has been suggested as a CO2 chemoreceptor site in mammals. Most of the studies involving the role of the LC in hypercapnic ventilatory responses have been performed in males. Since ovarian steroids modulate the activity of LC neurons and females have a different respiratory response to CO2 than males, we evaluated the activity of LC noradrenergic neurons during normocapnia and hypercapnia in female and male rats with distinct sex hormone levels. Ovariectomized (OVX), estradiol (E2)-treated ovariectomized (OVX+ E2) and female rats on the diestrous day of the estrous cycle were evaluated. Concurrently, males were investigated as gonad-intact, orchidectomized (ORX), testosterone (T)-treated ORX (ORX + T), and E2-treated ORX (ORX+ E2). Activation of LC neurons was determined by double-label immunohistochemistry to c-Fos and tyrosine hydroxylase (TH). Hypercapnia induced by 7% CO2 increased the number of c-Fos/THimmunoreactive (ir) neurons in the LC of all groups when compared to air exposure. Hypercapnia-induced c-Fos expression did not differ between diestrous females and intact male rats. In the OVX+ E2 group, there was attenuation in the c-Fos expression during normocapnia compared with OVX rats, butCO(2) responsiveness was not altered. Moreover, in ORX rats, neither T nor E2 treatments changed c-Fos expression in LC noradrenergic neurons. Thus, in female rats, E2 reduces activation of LC noradrenergic neurons, whereas in males, sex hormones do not influence the LC activity. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.