Imaging the Life and Death of mRNAs in Single Cells
Imaging the Life and Death of mRNAs in Single Cells
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DOI：
10.1101/cshperspect.a032086
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发表时间：
2018-12-01
影响因子：
7.2
通讯作者：
Lionnet, Timothee
中科院分区：
文献类型：
--
作者：
Chao, Jeffrey A.;Lionnet, Timothee
RNA在基因表达中起着核心作用，从细胞核中的转录到细胞质中的翻译和降解。荧光显微镜和标记方法的技术进步使得在固定和活细胞中检测RNA的单分子成为可能。在这里，我们专注于RNA成像的最新发展，它允许定量测量单个转录本从出生到死亡的生命以及单细胞和组织中的所有事件。对RNA在其天然细胞环境中的直接观察揭示了一个复杂的空间和时间调节层，这深刻影响了我们对RNA生物学的理解。
RNA plays a central role in gene expression from its transcription in the nucleus through translation and degradation in the cytoplasm. Technological advances in fluorescent microscopy and labeling methodologies have made it possible to detect single molecules of RNA in both fixed and living cells. Here, we focus on the recent developments in RNA imaging that have allowed quantitatively measuring the lives of individual transcripts from birth to death and all the events in between in single cells and tissues. Direct observation of RNAs within their native cellular environment has revealed a complex layer of spatial and temporal regulation that has profoundly impacted our understanding of RNA biology.