Suppression of suprachiasmatic nucleus neurone activity with a vasopressin receptor antagonist: possible role for endogenous vasopressin in circadian activity cycles in vitro
Suppression of suprachiasmatic nucleus neurone activity with a vasopressin receptor antagonist: possible role for endogenous vasopressin in circadian activity cycles in vitro
复制标题
用加压素受体拮抗剂抑制视交叉上核神经元活动：内源性加压素在体外昼夜节律活动周期中的可能作用
DOI：
10.1016/0304-3940(94)90943-1
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发表时间：
1994
影响因子：
2.5
通讯作者：
C. Ingram
中科院分区：
文献类型：
--
作者：
R. Mihai;T. S. Juss;C. Ingram
用[Arg8]加压素(AVP)及其受体拮抗剂[d(CH2)5，d-Tyr(OEt)2，Val4，Cit8]-加压素在体外对大鼠视交叉上核(SCN)神经元进行检测。52%的AVP反应神经元表现出拮抗剂诱导的活性降低，这表明存在内源性兴奋性音调。在主观亮相和暗相之间，这一效应的大小显著下降，这与内源性AVP兴奋的昼夜波动有助于SCN内的电活动周期的可能性一致。然而，在对拮抗剂敏感和不敏感的神经元中，在亮相和暗相之间观察到相似的基础活动波动，这表明内源性AVP不是决定昼夜周期的唯一因素。
Neurones of the rat suprachiasmatic nucleus (SCN) were tested with [Arg8]vasopressin (AVP) and the AVP receptor antagonist, [d(CH2)5,d-Tyr(OEt)2,Val4, Cit8]-vasopressin in vitro. 52% of AVP-responsive neurones showed an antagonist-induced decrease in activity, indicative of the presence of an endogenous excitatory tone. The magnitude of this effect declined significantly between subjective light and dark phases, consistent with the possibility that circadian fluctuations in endogenous AVP excitation contribute to the cycle of electrical activity within the SCN. However, similar fluctuations in basal activity between the light and dark phases was observed for both antagonist-sensitive and -insensitive neurones, indicating that endogenous AVP was not the only factor determining the circadian cycle.