Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1
Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1
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高度抗铬酸盐和还原性细菌菌株梭形赖氨酸芽孢杆菌 ZC1 的表征和基因组分析
DOI：
10.1016/j.jhazmat.2010.09.072
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发表时间：
2011-01-30
影响因子：
13.6
通讯作者：
Rensing, Christopher
中科院分区：
文献类型：
--
作者：
He, Minyan;Li, Xiangyang;Rensing, Christopher
从金属电镀厂铬（Cr）污染废水中分离得到的梭形赖氨酸芽孢杆菌ZC1在R2A培养基中的最小抑菌浓度（MIC）为60 mM，具有较高的耐铬（Cr（VI））能力。纺锤形乳杆菌ZC 1对多种金属（Cu、Ni、Co、Hg、Cd和Ag）和类金属（As）表现出抗性。该细菌表现出非常快速的Cr（VI）还原能力。在12 h内几乎完全还原1 mM K2CrO4。研究了I.醋酸钠和NADH可增强梭形菌ZC 1的活性。通过全基因组序列分析，发现菌株ZC1含有大量的金属（类）抗性基因。具体而言，一个chrA基因编码一个假定的铬酸盐转运赋予铬酸盐抗性被确定。在表型和基因表达分析中，铬酸盐抗性是组成性的。此外，我们还发现了一个yieF基因和几个编码还原酶的基因，它们可能参与了铬酸盐还原。相邻的推定的铬酸盐还原相关基因，nitR和yieF的表达，被认为是组成性的。大量的NADH依赖的铬酸盐还原酶基因可能负责快速的铬酸盐还原，以解毒Cr（VI），并在恶劣的废水环境中生存。(C)2010 Elsevier B.V.保留所有权利。
Lysinibacillus fusiformis ZC1 isolated from chromium (Cr) contaminated wastewater of a metal electroplating factory displayed high chromate [Cr(VI)] resistance with a minimal inhibitory concentration (MIC) of 60 mM in R2A medium. L fusiformis ZC1 showed resistances to multiple metals (Cu, Ni, Co, Hg, Cd and Ag) and a metalloid (As). This bacterium exhibited an extremely rapid Cr(VI) reduction capability. It almost completely reduced 1 mM K2CrO4 in 12 h. The Cr(VI) reduction ability of I. fusiformis ZC1 was enhanced by sodium acetate and NADH. By whole genome sequence analysis, strain ZC1 was found to contain large numbers of metal(loid) resistance genes. Specifically, a chrA gene encoding a putative chromate transporter conferring chromate resistance was identified. The chromate resistance was constitutive in both phenotypic and gene expression analyses. Furthermore, we found a yieF gene and several genes encoding reductases that were possibly involved in chromate reduction. Expression of adjacent putative chromate reduction related genes, nitR and yieF, was found to be constitutive. The large numbers of NADH-dependent chromate reductase genes may be responsible for the rapid chromate reduction in order to detoxify Cr(VI) and survive in the harsh wastewater environment. (C) 2010 Elsevier B.V. All rights reserved.