Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis
Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis
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DOI：
10.1104/pp.104.041012
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发表时间：
2004-08-01
期刊：
影响因子：
7.4
通讯作者：
Verpoorte, R
中科院分区：
文献类型：
--
作者：
Choi, YH;Tapias, EC;Verpoorte, R
使用一维和二维 NMR 光谱，然后进行主成分分析 (PCA)，对感染 10 种植原体的长春花 L. G. Don 进行了全面的代谢组学分析，这是一种无监督聚类方法，无需了解数据集，用于降低多变量数据的维数，同时保留其中的大部分方差。通过结合这些技术，我们能够识别出在植原体感染的玫瑰花叶子中与健康叶子中存在不同水平的代谢物。玫瑰花叶子中植原体的感染导致与苯丙素或萜类吲哚生物碱的生物合成途径相关的代谢物增加：绿原酸、马钱酸、塞洛甘宁和文多灵。此外，在植原体感染的叶子中检测到较高丰度的 Glc、Glu、多酚、琥珀酸和 Suc。健康和感染植原体的玫瑰花叶子的 H-1-NMR 信号的主成分分析表明，这些代谢物是区分植原体感染的玫瑰花叶子和健康叶子的主要区别因素。根据NMR和PCA分析，可能表明萜类吲哚生物碱以及苯丙素类的生物合成途径受到植原体感染的刺激。
A comprehensive metabolomic profiling of Catharanthus roseus L. G. Don infected by 10 types of phytoplasmas was carried out using one-dimensional and two-dimensional NMR spectroscopy followed by principal component analysis (PCA), an unsupervised clustering method requiring no knowledge of the data set and used to reduce the dimensionality of multivariate data while preserving most of the variance within it. With a combination of these techniques, we were able to identify those metabolites that were present in different levels in phytoplasma-infected C. roseus leaves than in healthy ones. The infection by phytoplasma in C. roseus leaves causes an increase of metabolites related to the biosynthetic pathways of phenylpropanoids or terpenoid indole alkaloids: chlorogenic acid, loganic acid, secologanin, and vindoline. Furthermore, higher abundance of Glc, Glu, polyphenols, succinic acid, and Suc were detected in the phytoplasma-infected leaves. The PCA of the H-1-NMR signals of healthy and phytoplasma-infected C. roseus leaves shows that these metabolites are major discriminating factors to characterize the phytoplasma-infected C. roseus leaves from healthy ones. Based on the NMR and PCA analysis, it might be suggested that the biosynthetic pathway of terpenoid indole alkaloids, together with that of phenylpropanoids, is stimulated by the infection of phytoplasma.