Processed lipoaspirate cells for tissue engineering of the lower urinary tract:: Implications for the treatment of stress urinary incontinence and bladder reconstruction
Processed lipoaspirate cells for tissue engineering of the lower urinary tract:: Implications for the treatment of stress urinary incontinence and bladder reconstruction
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DOI：
10.1097/01.ju.0000176489.96993.84
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发表时间：
2005-11-01
影响因子：
6.6
通讯作者：
Rodríguez, LV
中科院分区：
文献类型：
--
作者：
Jack, GS;Almeida, FG;Rodríguez, LV
目的：我们进行了一项试点研究，以探讨人类脂肪来源的，多能干细胞的能力，被交付到膀胱和尿道smoothmuscles.Materials和方法内生存：Lipoaspirate从女性患者接受吸脂术。处理脂肪抽吸物以产生经处理的脂肪抽吸物（PLA）细胞的多能群体。对于组织递送，将PLA细胞荧光标记并悬浮于Hanks平衡盐溶液（Sigma Chemical Co.，圣路易斯，密苏里州）。为了评估PLA在多种动物模型中的存活率，对8只Rnu无胸腺大鼠（Charles River，威尔明顿，马萨诸塞州）和6只SCID小鼠（Taconic Farms，奥克斯纳德，加州）进行剖腹手术并将PLA细胞注射到膀胱和尿道中。另外8只大鼠仅接受Hanks平衡盐溶液的假注射。实验组和对照组动物分别于注射后2、4、8和12周处死，取膀胱和尿道组织进行分析。结果：从人脂肪组织中分离出了具有自我再生能力的多能PLA细胞。注射后2、4、8和12周的评价证实了PLA细胞活力和掺入受体平滑肌中。注射后8周，PLA细胞表现出在体内表达的α-平滑肌肌动蛋白，平滑肌分化的早期标志物。结论：PLA细胞是一种容易获得的多能细胞来源，使它们成为理想的组织再生。PLA细胞在下尿路中可存活长达12周。注射到泌尿道中的人PLA细胞显示出随时间推移平滑肌掺入和分化的形态学和表型证据。PLA细胞可能为尿路重建提供一种可行且经济的细胞来源。
Purpose: We performed a pilot study to investigate the ability of human adipose derived, multipotent stem cells to be delivered to and survive within bladder and urethral smooth muscle.Materials and Methods: Lipoaspirate was acquired from female patients undergoing liposuction. The lipoaspirate was processed to yield a pluripotent population of processed lipoaspirate (PLA) cells. For tissue delivery PLA cells were fluorescent labeled and suspended in Hanks' balanced salt solution (Sigma Chemical Co., St. Louis, Missouri). To assess PLA viability in multiple animal models 8 Rnu athymic rats (Charles River, Wilmington, Massachusetts) and 6 SCID mice (Taconic Farms, Oxnard, California) underwent laparotomy and injection of PLA cells into the bladder and urethra. An additional 8 rats underwent sham injection of Hanks' balanced salt solution alone. Experimental and control animals were sacrificed 2, 4, 8 and 12 weeks after injection, and the bladders and urethras were analyzed.Results: Self-regenerating, pluripotent PLA cells were easily isolated from human adipose tissue. Evaluation 2, 4, 8 and 12 weeks after injection demonstrated PLA cell viability and incorporation into the recipient smooth muscle. Eight weeks following injection PLA cells demonstrated in vivo expression of a-smooth muscle actin, an early marker of smooth muscle differentiation.Conclusions: PLA cells are an easily accessible source of pluripotent cells, making them ideal for tissue regeneration. PLA cells remain viable up to 12 weeks in the lower urinary tract. Human PLA cells injected into the urinary tract show morphological and phenotypic evidence of smooth muscle incorporation and differentiation with time. PLA cells may provide a feasible and cost-effective cell source for urinary tract reconstruction.