Variation in the transcriptome of different ecotypes of Arabidopsis thaliana reveals signatures of oxidative stress in plant responses to spaceflight
Variation in the transcriptome of different ecotypes of Arabidopsis thaliana reveals signatures of oxidative stress in plant responses to spaceflight
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DOI：
10.1002/ajb2.1223
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发表时间：
2019-01-01
影响因子：
3
通讯作者：
Gilroy, Simon
中科院分区：
文献类型：
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作者：
Choi, Won-Gyu;Barker, Richard J.;Gilroy, Simon
太空飞行提供了一个独特的环境，在其中剖析植物的应激反应行为，并揭示潜在的新途径在太空中触发。因此，我们分析了在国际空间站上生长的拟南芥植物的转录组，以找到这些与空间相关的反应网络的分子指纹。方法在轨道上培养四种生态型（Col-0、Ws-2、Ler-0和Cvi-0），然后使用RNA测序将它们的转录丰度模式与地面对照进行比较。关键结果热休克蛋白的转录本在太空飞行中的所有生态型中上调，而过氧化物酶转录本下调。在共享和生态类型的具体变化，与氧化应激和缺氧相关的基因类进行了检测。这些太空飞行转录反应特征可以通过低氧环境在地球上部分模仿，而通过氧化应激（H2 O2）处理则可以更充分地模仿。结论这些结果表明，航天环境与氧化应激可能引发的，部分是由缺氧反应。此外，一个共享的航天反应可能是通过诱导分子伴侣（如热休克蛋白），帮助保护细胞机制免受氧化损伤的影响。此外，这项研究强调了在设计和解释与航天实验有关的变化时考虑自然变化影响的重要性。
Premise of the Study Spaceflight provides a unique environment in which to dissect plant stress response behaviors and to reveal potentially novel pathways triggered in space. We therefore analyzed the transcriptomes of Arabidopsis thaliana plants grown on board the International Space Station to find the molecular fingerprints of these space-related response networks. Methods Four ecotypes (Col-0, Ws-2, Ler-0 and Cvi-0) were grown on orbit and then their patterns of transcript abundance compared to ground-based controls using RNA sequencing. Key Results Transcripts from heat-shock proteins were upregulated in all ecotypes in spaceflight, whereas peroxidase transcripts were downregulated. Among the shared and ecotype-specific changes, gene classes related to oxidative stress and hypoxia were detected. These spaceflight transcriptional response signatures could be partly mimicked on Earth by a low oxygen environment and more fully by oxidative stress (H2O2) treatments. Conclusions These results suggest that the spaceflight environment is associated with oxidative stress potentially triggered, in part, by hypoxic response. Further, a shared spaceflight response may be through the induction of molecular chaperones (such as heat shock proteins) that help protect cellular machinery from the effects of oxidative damage. In addition, this research emphasizes the importance of considering the effects of natural variation when designing and interpreting changes associated with spaceflight experiments.