Large-scale cell production of stem cells for clinical application using the automated cell processing machine.
Large-scale cell production of stem cells for clinical application using the automated cell processing machine.
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DOI：
10.1186/1472-6750-13-102
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发表时间：
2013-11-15
影响因子：
3.5
通讯作者：
Gojo S
中科院分区：
文献类型：
--
作者：
Kami D;Watakabe K;Yamazaki-Inoue M;Minami K;Kitani T;Itakura Y;Toyoda M;Sakurai T;Umezawa A;Gojo S
基于细胞的再生疗法在临床医学的新领域中具有巨大的应用潜力，尽管对于广泛的临床应用仍存在一些障碍有待克服。一个主要的障碍是难以大规模生产具有再现性的感兴趣的细胞。目前的细胞治疗方案需要耗时费力的手动过程。为了解决这个问题，我们专注于自动化和高通量细胞培养系统的机器人技术。迄今为止，尚未报道在临床试验中自动机器人培养干细胞或祖细胞。本研究中使用的AutoCulture®系统可以自动更换培养基、离心细胞、分裂细胞并拍摄照片进行形态学评估。我们研究了该系统在临床环境中的可行性。我们观察到两种培养方法在生长速率、基因表达谱、荧光激活细胞分选的细胞表面谱、表面聚糖谱和基因组DNA稳定性方面具有相似的特征。这些结果表明，AutoCulture®是一种用于再生医学的人类细胞培养的可行方法。自动化细胞处理机将在细胞治疗中发挥重要作用，并从多中心试验的应用到提供现成的细胞产品都有广泛的用途。
Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One major impediment is the difficulty in large-scale production of cells of interest with reproducibility. Current protocols of cell therapy require a time-consuming and laborious manual process. To solve this problem, we focused on the robotics of an automated and high-throughput cell culture system. Automated robotic cultivation of stem or progenitor cells in clinical trials has not been reported till date. The system AutoCulture® used in this study can automatically replace the culture medium, centrifuge cells, split cells, and take photographs for morphological assessment. We examined the feasibility of this system in a clinical setting. We observed similar characteristics by both the culture methods in terms of the growth rate, gene expression profile, cell surface profile by fluorescence-activated cell sorting, surface glycan profile, and genomic DNA stability. These results indicate that AutoCulture® is a feasible method for the cultivation of human cells for regenerative medicine. An automated cell-processing machine will play important roles in cell therapy and have widespread use from application in multicenter trials to provision of off-the-shelf cell products.