EFFECT OF SPECIFIC HUMORAL IMMUNITY AND SOME NON-SPECIFIC FACTORS ON RESISTANCE OF VOLUNTEERS TO RESPIRATORY CORONAVIRUS INFECTION
EFFECT OF SPECIFIC HUMORAL IMMUNITY AND SOME NON-SPECIFIC FACTORS ON RESISTANCE OF VOLUNTEERS TO RESPIRATORY CORONAVIRUS INFECTION
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DOI：
10.1017/s0022172400062410
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发表时间：
1985-01-01
影响因子：
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通讯作者：
CALLOW, KA
中科院分区：
文献类型：
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作者：
CALLOW, KA
33名志愿者鼻腔接种冠状病毒229E，通过抗体升高、症状学和病毒排泄监测他们的反应。这与他们试验前的免疫状态有关，如血清和鼻腔冲洗液中特定抗体和非特定蛋白的浓度所表明的。循环和局部特异性抗体都与预防感染和疾病有关，但只有这两种类型的特异性IgA抗体似乎都能缩短病毒脱落的时间。虽然总分泌型IgA只与症状的减轻显著相关，但鼻洗液中的总蛋白似乎也能预防感染，这表明当地产生的其他蛋白(未鉴定)可能与耐药性有关。在可能影响循环和局部保护蛋白浓度从而影响病毒接种结果的许多因素中，有两个因素，即志愿者的性别和自上次感冒以来的间隔时间进行了检查。男性志愿者或最近有呼吸道感染证据的志愿者感染的可能性较小，但如果他们被感染，他们的临床评分较低，并且比其他人更早停止传播病毒。这些组在其挑战前的血清和/或鼻液中具有更高浓度的特异性抗体和非特异性蛋白。讨论了这些发现的意义。
Thirty-three volunteers were inoculated intranasally with coronavirus 229E, and their responses monitored by antibody rises, symptomatology and virus excretion. These were related to their pre-trial immune status as indicated by concentrations of specific antibodies and non-specific proteins in serum and nasal washings. Both circulating and local specific antibodies were associated with protection from infection and disease, but only specific IgA antibodies of either type appeared to shorten the period of virus shedding. Although total secretory IgA was significantly associated only with reduction of symptoms, total protein in nasal washings appeared to protect against infection also, indicating that other locally produced proteins, not identified, may be associated with resistance. Two of the many factors which may affect the concentration of circulating and local protective proteins and thus influence the outcome of virus inoculation, namely, sex of the volunteer and the interval since the previous cold, were examined. Male volunteers or volunteers who had had evidence of a recent respiratory infection were less likely to be infected, but if they were infected, they had lower clinical scores and stopped shedding virus earlier than the rest. These groups possessed higher concentrations of specific antibodies and non-specific proteins in their pre-challenge sera and/or nasal washings. The significance of these findings is discussed.