Monitoring changes in anthocyanin and steroid alkaloid glycoside content in lines of transgenic potato plants using liquid chromatography/mass spectrometry
Monitoring changes in anthocyanin and steroid alkaloid glycoside content in lines of transgenic potato plants using liquid chromatography/mass spectrometry
复制标题
DOI：
10.1016/s0031-9422(02)00720-3
复制
发表时间：
2003-03-01
期刊：
影响因子：
3.8
通讯作者：
Szopa, J
中科院分区：
文献类型：
--
作者：
Stobiecki, M;Matysiak-Kata, W;Szopa, J
建立了过表达和抑制类黄酮生物合成酶的转基因马铃薯植株，并对其进行了分析。所选植物在花青素合成水平上明显表现出预期的变化。编码二氢黄酮醇4-还原酶（DFR）的DNA在意义取向上的过表达导致了薯类花青素的增加，矮牵牛花苷和天龙花苷衍生物的含量增加了4倍。用相应的反义结构转化植株时，花青素水平显著降低。马铃薯植株的转化还伴随着转基因马铃薯块茎中甾体生物碱苷（SAG）水平的显著变化。转基因马铃薯中SAGs含量的变化不依赖于黄酮类成分。然而，在检测到最高（DFR11）或最低（DFRa3）花青素水平的极端情况下，与类固醇生物碱含量的正相关明显可见。分析认为，SAGs含量的变化与转化过程中染色质受到胁迫有关。采用电喷雾电离液相色谱/质谱（LC/MS）系统对12个转基因马铃薯品系块茎中甾体生物碱苷的定性和定量变化进行了分析。除在干块茎皮提取物中鉴定出少量的-茄碱和-茄碱外，还鉴定出茄碱脱氢二聚体的甘油三酯。2003爱思唯尔科学有限公司版权所有。
Transgenic potato plants overexpressing and repressing enzymes of flavonoids biosynthesis were created and analyzed. The selected plants clearly showed the expected changes in anthocyanins synthesis level. Overexpression of a DNA encoding dihydroflavonol 4-reductase (DFR) in sense orientation resulted in an increase in tuber anthocyanins, a 4-fold increase in petunidin and pelargonidin derivatives. A significant decrease in anthocyanin level was observed when the plant was transformed with a corresponding antisense construct. The transformation of potato plants was also accompanied by significant changes in steroid alkaloid glycosides (SAG) level in transgenic potato tuber. The changes in SAGs content was not dependent on flavonoid composition in transgenic potato. However, in an extreme situation where the highest (DFR11) or the lowest (DFRa3) anthocyanin level was detected the positive correlation with steroid alkaloid content was clearly visible. It is suggested that the changes in SAGs content resulted from chromatin stressed upon transformation. A liquid chromatography/mass spectrometry (LC/MS) system with electrospray ionization was applied for profiling qualitative and quantitative changes of steroid alkaloid glycosides in tubers of twelve lines of transgenic potato plants. Except alpha-chaconine and alpha-solanine, in the extracts from dried tuber skin alpha-solamargine and alpha-solasonine, triglycosides of solasonine, were identified in minor amounts, triglycosides of solanidine dehydrodimers were also recognized. (C) 2003 Elsevier Science Ltd. All rights reserved.