Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol–disulfide exchange
Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol–disulfide exchange
复制标题
制备细胞培养玻璃表面，通过硫醇-二硫化物交换触发释放支化聚乙烯亚胺
DOI：
10.1016/j.colsurfb.2012.10.031
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Aya Mizutani Akimoto
中科院分区：
文献类型：
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作者：
坂野 貴宣;弓場 英司;原田 敦史;河野 健司;石原俊也,鄭雄一,酒井崇匡;Aya Mizutani Akimoto
为了开发一种用于细胞培养的化学刺激响应性底物，将含有吡啶二硫部分的聚乙烯亚胺(PEI)通过二硫键连接到用含硫醇基的硅烷偶联剂修饰的玻璃盖片上。用X-射线光电子能谱和Zeta电位分析对其进行了表面改性。得到的表面具有足够高的细胞粘附性。Zeta电位测量表明，当修饰后的玻璃盖片浸泡在含有半胱氨酸的中性pH缓冲液中时，PEI衍生物通过硫醇-二硫键交换从表面释放出来。细胞存活率测定表明，该化学刺激对293T细胞基本无毒。由于PEI是一种广泛使用的转染剂，这种功能性玻璃盖片将有可能成为反向转染法的实验平台。
To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol–disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.