Mining the Human Proteome: Biomarker Discovery for Human Cancer and Metastases.
Mining the Human Proteome: Biomarker Discovery for Human Cancer and Metastases.
复制标题
挖掘人类蛋白质组：人类癌症和转移的生物标志物发现。
DOI：
10.1097/ppo.0000000000000139
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Moses,MarshaA
中科院分区：
文献类型：
--
作者：
Yang,Jiang;Roy,Roopali;Jedinak,Andrej;Moses,MarshaA
随着我们对癌症发生和发展机制的了解不断增加，更有效、毒性更小的靶向治疗方法也开始进入临床。然而，这些临床进展的全面影响和精准医学新兴领域的实际成功取决于敏感和准确的生物标志物的发现和验证，这些生物标志物可以实现适当和严格的样本类型和患者选择，可靠的治疗效果纵向监测，甚至风险评估和早期检测。在本综述的背景下，我们研究了发现和验证非侵入性癌症生物标志物的最新方法，特别强调那些蛋白质或蛋白质相关的生物标志物。我们还回顾了样品选择策略，目前用于发现和验证要求的蛋白质组学方法以及数据分析标准。最后，我们从我们自己的生物标志物研究中提供了这些元素的发现和验证的例子。
As our knowledge of the mechanisms underlying cancer development and progression has increased, so too have more effective, less toxic, and targeted therapies begun to reach the clinic. However, the full impact of these clinical advances and the practical success of the emerging field of precision medicine are dependent on the discovery and validation of sensitive and accurate biomarkers that can enable appropriate and rigorous sample type and patient selection, reliable longitudinal monitoring of therapeutic efficacy, and even risk assessment and early detection. Within the context of this review, we examine state-of-the-art approaches to the discovery and validation of noninvasive cancer biomarkers, with a specific emphasis on those that are protein or protein-associated ones. We also review sample selection strategies, currently utilized proteomic approaches for both discovery and validation requirements, and data analysis standards. Finally, we provide examples of these elements of biomarker discovery and validation from our own biomarker research.