PAS domain-mediated WC-1/WC-2 interaction is essential for maintaining the steady-state level of WC-1 and the function of both proteins in circadian clock and light responses of Neurospora
PAS domain-mediated WC-1/WC-2 interaction is essential for maintaining the steady-state level of WC-1 and the function of both proteins in circadian clock and light responses of Neurospora
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DOI：
10.1128/mcb.22.2.517-524.2002
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发表时间：
2002-01-01
影响因子：
5.3
通讯作者：
Liu, Y
中科院分区：
文献类型：
--
作者：
Cheng, P;Yang, YH;Liu, Y
在基于frq-we的脉孢菌昼夜节律反馈环中，两个含有PAS结构域的转录因子白色COLLAR-1（WC-1）和WC-2形成异二聚体复合物，激活频率（frq）的转录。FRQ在这些反馈回路中起两个作用：通过与WC复合物相互作用抑制其自身的转录，并正性上调WC-1和WC-2蛋白的水平。我们在这里报告，WC-1蛋白的稳态水平是独立的FRQ和WC-2通过不同的转录后机制进行调节。WC-1水平在wc-2敲除菌株中极低，并且这种低表达水平不依赖于wc-1转录和FRQ蛋白表达。此外，我们的数据表明，PAS结构域的WC-2介导的相互作用，这种蛋白质与WC-1和FRQ在体内。这种相互作用对于维持WC-1的稳态水平以及WC-1和WC-2在生物钟和光响应中的适当功能是必不可少的。
In the frq-we-based circadian feedback loops of Neurospora, two PAS domain-containing transcription factors, WHITE COLLAR-1 (WC-1) and WC-2, form heterodimeric complexes that activate the transcription of frequency (frq). FRQ serves two roles in these feedback loops: repressing its own transcription by interacting with the WC complex and positively upregulating the levels of WC-1 and WC-2 proteins. We report here that the steady-state level of WC-1 protein is independently regulated by both FRQ and WC-2 through different posttranscriptional mechanisms. The WC-1 level is extremely low in wc-2 knockout strains, and this low level of expression is independent of wc-1 transcription and FRQ protein expression. In addition, our data show that the PAS domain of WC-2 mediates the interactions of this protein with both WC-1 and FRQ in vivo. Such interactions are essential for maintaining the steady-state level of WC-1 and the proper function of WC-1 and WC-2 in circadian clock and light responses.