Aerobic and anaerobic respiratory systems in Campylobacter fetus subsp. jejuni grown in atmospheres containing hydrogen.
Aerobic and anaerobic respiratory systems in Campylobacter fetus subsp. jejuni grown in atmospheres containing hydrogen.
复制标题
胎儿弯曲杆菌亚种的需氧和厌氧呼吸系统。
DOI：
10.1128/jb.152.1.306-314.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
Lascelles,J
中科院分区：
文献类型：
--
作者：
Carlone,GM;Lascelles,J
胎儿弯曲杆菌亚种的最大生长。当培养物在含有约30%氢气、5%氧气和10%CO2的气氛中振荡孵育时，发生空肠弯曲菌菌株C-61。在这些条件下生长的细胞悬浮液在存在0.33mM氰化物的情况下以甲酸盐作为底物消耗氧，所述氰化物用抗坏血酸-N，N，N ′，N ′-四甲基-对苯二胺和乳酸盐完全抑制呼吸。完整细胞的光谱证据表明，一种形式的细胞色素c，可与甲酸盐还原，但不能与乳酸盐或抗坏血酸盐-N，N，N '，N'-四甲基-对苯二胺，可以再氧化的氰化物不敏感的系统。对细胞膜的分析表明，细胞色素c、细胞色素B和各种酶（包括氢化酶、甲酸脱氢酶和延胡索酸还原酶）的高电位和低电位形式。主要的一氧化碳结合色素似乎是细胞色素c的一种形式，但光谱也显示出细胞色素o的证据。在2-庚基-4-羟基喹啉-N-氧化物的存在下，膜细胞色素被氢还原，其浓度防止了以琥珀酸酯为电子供体的细胞色素c的还原。再氧化的底物还原的细胞色素由氧显然介导的氰化物敏感和氰化物不敏感的系统。该膜还具有由细胞色素B介导的富马酸氢氧化还原酶活性。结果表明，C.胎儿空肠具有与复杂的末端氧化酶系统相关的高电位和低电位形式的细胞色素。
Maximum growth of Campylobacter fetus subsp. jejuni, strain C-61, occurred when the cultures were incubated with shaking in atmospheres containing approximately 30% hydrogen, 5% oxygen, and 10% CO2. Suspensions of cells grown under these conditions consumed oxygen with formate as the substrate in the presence of 0.33 mM cyanide, which completely inhibited respiration with ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine and with lactate. Spectroscopic evidence with intact cells suggested that a form of cytochrome c, reducible with formate but not with lactate or ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine, can be reoxidized by a cyanide-insensitive system. Analysis of membranes from the cells showed high- and low-potential forms of cytochrome c, cytochrome b, and various enzymes, including hydrogenase, formate dehydrogenase, and fumarate reductase. The predominant carbon monoxide-binding pigment appeared to be a form of cytochrome c, but the spectra also showed evidence of cytochrome o. The membrane cytochromes were reduced by hydrogen in the presence of 2-heptyl-4-hydroxyquinoline-N-oxide at concentrations which prevented the reduction of cytochrome c with succinate as the electron donor. Reoxidation of the substrate-reduced cytochromes by oxygen was apparently mediated by cyanide-sensitive and cyanide-insensitive systems. The membranes also had hydrogen-fumarate oxidoreductase activity mediated by cytochrome b. We conclude that C. fetus jejuni has high- and low-potential forms of cytochrome which are associated with a complex terminal oxidase system.