Inter-laboratory variability in in vitro spinal segment flexibility testing
Inter-laboratory variability in in vitro spinal segment flexibility testing
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DOI：
10.1016/j.jbiomech.2011.06.034
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发表时间：
2011-09-02
影响因子：
2.4
通讯作者：
Bechtold, Joan E.
中科院分区：
文献类型：
--
作者：
Wheeler, Daniel J.;Freeman, Andrew L.;Bechtold, Joan E.
使用各种实验室专用的加载装置和条件进行体外脊柱柔韧性测试，使得实验室之间的测试结果难以比较。纯力矩的应用已被广泛应用于脊柱柔韧性测试，但据我们所知，尚未尝试量化实验室特定加载装置导致的活动度（ROM）差异。在四个独立的实验室对七个新鲜冷冻的腰椎尸体运动节段进行了完整的测试。在没有轴向预载荷的情况下，在每个解剖平面上施加7.5 Nm的无约束纯力矩。在实验室A和B，使用带有被动（A）或受控（B）XY工作台的液压驱动脊柱加载夹具施加纯力矩。在实验室C和D，使用滑动（C）或固定环（D）电缆滑轮系统和伺服液压试验架施加纯力矩。在实验室A和B应用三个正弦加载-卸载循环，而在实验室C和D应用一个准静态循环，增量为1.5 Nm。非接触式运动测量系统被用来量化ROM。在所有的测试方向，捐助者之间的ROM变异性大于单捐助者ROM实验室之间的变异性。任意两个实验室之间的平均ROM的最大差异为屈曲-伸展1.5度，侧弯1.3度和轴向扭转1.1度。这是第一项在四个独立实验室采用不同的纯力矩系统对一组脊柱运动节段的ROM进行量化的研究。这些数据支持我们的假设，即给定一个描述良好的测试方法，独立的实验室可以产生类似的生物力学结果。(C)2011爱思唯尔有限公司保留所有权利。
In vitro spine flexibility testing has been performed using a variety of laboratory-specific loading apparatuses and conditions, making test results across laboratories difficult to compare. The application of pure moments has been well established for spine flexibility testing, but to our knowledge there have been no attempts to quantify differences in range of motion (ROM) resulting from laboratory-specific loading apparatuses. Seven fresh-frozen lumbar cadaveric motion segments were tested intact at four independent laboratories. Unconstrained pure moments of 7.5 Nm were applied in each anatomic plane without an axial preload. At laboratories A and B, pure moments were applied using hydraulically actuated spinal loading fixtures with either a passive (A) or controlled (B) XY table. At laboratories C and D, pure moments were applied using a sliding (C) or fixed ring (D) cable-pulley system with a servohydraulic test frame. Three sinusoidal load-unload cycles were applied at laboratories A and B while a single quasistatic cycle was applied in 1.5 Nm increments at laboratories C and D. Non-contact motion measurement systems were used to quantify ROM. In all test directions, the ROM variability among donors was greater than single-donor ROM variability among laboratories. The maximum difference in average ROM between any two laboratories was 1.5 degrees in flexion-extension, 1.3 degrees in lateral bending and 1.1 degrees in axial torsion. This was the first study to quantify ROM in a single group of spinal motion segments at four independent laboratories with varying pure moment systems. These data support our hypothesis that given a well-described test method, independent laboratories can produce similar biomechanical outcomes. (C) 2011 Elsevier Ltd. All rights reserved.