Laser-stimulated fluorescence of submicrometer regions within single mitochondria of rhodamine-treated myocardial cells in culture.
Laser-stimulated fluorescence of submicrometer regions within single mitochondria of rhodamine-treated myocardial cells in culture.
复制标题
培养中经罗丹明处理的心肌细胞单个线粒体内亚微米区域的激光刺激荧光。
DOI：
10.1073/pnas.79.2.466
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发表时间：
1982
影响因子：
11.1
通讯作者：
Berns,MW
中科院分区：
文献类型：
--
作者：
Siemens,A;Walter,R;Liaw,LH;Berns,MW
使用442 nm的聚焦激光束在培养的心肌和内皮细胞的单个线粒体中的0.5微米斑点中激发荧光。用罗丹明6G或123处理细胞以使线粒体发荧光。罗丹明123处理的细胞在几分钟内表现出荧光逐渐减少，而罗丹明6G处理的心肌细胞表现出三种不同的模式的可变荧光强度。这些模式在同一线粒体或不同线粒体内的不同点被检测到。来自非心肌内皮细胞的线粒体没有表现出任何可变的荧光强度模式。电子显微镜显示没有超微结构损伤归因于激光照射的线粒体。可变的荧光模式被假设为指示在亚细胞器水平上的分子或离子的局部改变。
A focused laser beam of 442 nm was used to stimulate fluorescence in 0.5-micrometer spots in single mitochondria of myocardial and endothelial cells in culture. Cells were treated with rhodamine 6G or 123 in order to render the mitochondria fluorescent. Rhodamine 123-treated cells exhibited a gradual decrease in fluorescence over several minutes, whereas the rhodamine 6G-treated myocardial cells exhibited three distinct patterns of variable fluorescence intensity. These patterns were detected at different points within the same mitochondrion or in different mitochondria. Mitochondria from nonmyocardial endothelial cells did not exhibit any variable intensity patterns of fluorescence. Electron microscopy revealed no ultrastructural damage attributable to laser exposure of the mitochondria. The variable fluorescence patterns are hypothesized to be indicative of localized alterations in molecules or ions at the suborganelle level.