50-Hertz electromagnetic fields induce gammaH2AX foci formation in mouse preimplantation embryos in vitro
50-Hertz electromagnetic fields induce gammaH2AX foci formation in mouse preimplantation embryos in vitro
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DOI：
10.1095/biolreprod.106.052241
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发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Huang, He-Feng
中科院分区：
文献类型：
--
作者：
Luo, Qiong;Yang, Jun;Huang, He-Feng
电磁场对哺乳动物DNA损伤的影响仍存在争议。本研究采用检测DNA双链断裂（DSBs）的新敏感指标gammaH2AX病灶形成，研究了电磁场对体外植入前小鼠胚胎DNA损伤的影响。实验结果表明，电磁场能降低小鼠胚胎的卵裂率。电磁场的这种降低作用与诱导着床前小鼠胚胎中gammaH2AX病灶形成所表明的dna损伤作用有关。噪声电磁场或wortmannin（一种磷脂酰肌醇3-激酶（PI3K）家族抑制剂）可抑制电磁场对gammaH2AX灶形成的诱导作用。此外，获得的数据还表明，电磁场可以通过将修复因子Rad50招募到受损的DNA位点来修复相应的DNA损伤，从而激活DNA损伤修复机制。这些发现表明，电磁场可能导致体外植入前胚胎的DNA损伤，并且电磁场对发育的不利影响可能至少部分通过DNA损伤起作用。电磁场引起的DNA损伤至少部分可以通过DNA损伤修复机制的自然激活修复，或者通过同时处理噪声磁场来防止。
Effects of electromagnetic fields (EMFs) on DNA damage in mammals are still controversial. In the present study, the effects of EMFs on DNA damage in preimplantation mouse embryos in vitro were investigated by using gammaH2AX foci formation, a new sensitive indicator for detecting DNA double-strand breaks (DSBs). The data obtained demonstrated that EMFs decreased the cleavage rate of preimplantation mouse embryos. This decreasing effect of EMFs was related to the DNA-damaging effect indicated by the induction of gammaH2AX foci formation in preimplantation mouse embryos. The inducing effects of EMFs on gammaH2AX foci formation could be inhibited by the treatment of noise MFs or wortmannin, a phosphatidylinositol 3-kinase (PI3K) family inhibitor. Furthermore, the data obtained also showed that EMFs could activate the DNA damage-repair mechanism by recruiting repair factor Rad50 to the damaged DNA sites to repair the corresponding DNA damage. These findings suggest that EMFs could cause DNA damage in preimplantation embryos in vitro and that the adverse effects of EMFs on development might at least partly act through DNA damage. The DNA damage induced by EMFs could be at least partly repaired by the natural activation of DNA damage-repair mechanism or prevented by the simultaneous treatment of noise magentic fields.