Novel kruppel-like factor is induced by neuronal activity and by sensory input in the central nervous system of the terrestrial slug Limax valentianus
Novel kruppel-like factor is induced by neuronal activity and by sensory input in the central nervous system of the terrestrial slug Limax valentianus
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DOI：
10.1002/neu.20210
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发表时间：
2006-02-01
期刊：
影响因子：
--
通讯作者：
Kirino, Y
中科院分区：
文献类型：
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作者：
Fukunaga, S;Matsuo, R;Kirino, Y
在蜥蜴中枢神经系统中，前大脑被认为是气味信息处理和气味记忆保留的场所，但对该学习方案中使用的有害刺激的输入途径知之甚少。为了深入了解参与记忆形成的有害刺激的感觉信息处理，我们筛选了人工神经元活动诱导的基因，并鉴定了一种克鲁佩尔样因子（KLF）家族转录因子基因（Limax KLF；limKLF），该基因在去极化后30分钟上调。与 GFP 融合的 limKLF 蛋白定位于 COS-7 细胞的细胞核。我们还通过逆转录聚合酶链反应（RT-PCR）克隆了Limax的立即早期基因CCAAT增强子结合蛋白（C/EBP）。通过以不依赖于蛋白质合成的方式将蛞蝓的中枢神经系统（CNS）解剖出来，以及对蛞蝓身体的各种有害刺激，这两个基因都会上调。这些基因可用作 Limax 中的神经元活动标记，以可视化激活的感觉神经系统。 (c) 2005 年 Wiley 期刊公司。
In the Limax central nervous system, the procerebrum is thought to be the locus of odor information processing and odor memory retention, but little is known about the input pathway of the noxious stimuli used in this learning protocol. To gain insight into the sensory information processing of the noxious stimuli involved in memory formation, we screened genes induced by artificial neuronal activity, and identified one kruppel-like factor (KLF) family transcription factor gene (Limax KLF; limKLF), which is up-regulated 30 min after depolarization. The limKLF protein fused to GFP was localized to the nucleus in COS-7 cells. We also cloned an immediate early gene, CCAAT enhancer binding protein (C/EBP), of Limax by reverse transcription-polymerase chain reaction (RT-PCR). Both genes were up-regulated by dissection of the central nervous system (CNS) out of the slug in a protein synthesis-independent manner, and also by various noxious stimuli to the slug's body. These genes may be useful as neuronal activity markers in Limax to visualize activated sensory nervous systems. (c) 2005 Wiley Periodicals, Inc.