In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals
In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals
复制标题
激素和化学物质促进胰岛簇的体外扩张
DOI：
10.1038/s41421-020-0159-x
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发表时间：
2020-04
期刊：
影响因子：
33.5
通讯作者：
Yu Xiao
中科院分区：
文献类型：
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作者：
Lin Jing-Yu;Cheng Jie;Du Ya-Qin;Pan Wei;Zhang Zhong;Wang Jin;An Jie;Yang Fan;Xu Yun-Fei;Lin Hui;An Wen-Tao;Wang Jia;Yang Zhao;Chai Ren-Jie;Sha Xue-Ying;Hu Hui-Li;Sun Jin-Peng;Yu Xiao
组织再生，比如胰岛组织在体外的增殖，能够作为糖尿病治疗和个性化药物测试的一种有前景的策略。然而，这样一种策略尚未实现。增殖可分为两个阶段。
Tissue regeneration, such as pancreatic islet tissue propagation in vitro, could serve as a promising strategy for diabetes therapy and personalised drug testing. However, such a strategy has not been realised yet. Propagation could be divided into two steps, in vitro expansion and repeated passaging. Even the first step of the in vitro islet expansion has not been achieved to date. Here, we describe a method that enables the expansion of islet clusters isolated from pregnant mice or wild-type rats by employing a combination of specific regeneration factors and chemical compounds in vitro. The expanded islet clusters expressed insulin, glucagon and somatostatin, which are markers corresponding to pancreatic β cells, α cells and δ cells, respectively. These different types of cells grouped together, were spatially organised and functioned similarly to primary islets. Further mechanistic analysis revealed that forskolin in our recipe contributed to renewal and regeneration, whereas exendin-4 was essential for preserving islet cell identity. Our results provide a novel method for the in vitro expansion of islet clusters, which is an important step forward in developing future protocols and media used for islet tissue propagation in vitro. Such method is important for future regenerative diabetes therapies and personalised medicines using large amounts of pancreatic islets derived from the same person.
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影响因子：
23.9
作者：
Li K;Zhu S;Russ HA;Xu S;Xu T;Zhang Y;Ma T;Hebrok M;Ding S
通讯作者：
Ding S
影响因子：
7.7
作者：
Lebensohn AM;Rohatgi R
通讯作者：
Rohatgi R
影响因子：
64.5
作者：
Huch M;Gehart H;van Boxtel R;Hamer K;Blokzijl F;Verstegen MM;Ellis E;van Wenum M;Fuchs SA;de Ligt J;van de Wetering M;Sasaki N;Boers SJ;Kemperman H;de Jonge J;Ijzermans JN;Nieuwenhuis EE;Hoekstra R;Strom S;Vries RR;van der Laan LJ;Cuppen E;Clevers H
通讯作者：
Clevers H
影响因子：
16.6
作者：
Zhu S;Russ HA;Wang X;Zhang M;Ma T;Xu T;Tang S;Hebrok M;Ding S
通讯作者：
Ding S
影响因子：
82.9
作者：
通讯作者：
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