Detection of IgM and IgG antibodies in patients with coronavirus disease 2019
Detection of IgM and IgG antibodies in patients with coronavirus disease 2019
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DOI：
10.1002/cti2.1136
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发表时间：
2020-05-01
影响因子：
5.8
通讯作者：
Sun, Ziyong
中科院分区：
文献类型：
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作者：
Hou, Hongyan;Wang, Ting;Sun, Ziyong
目的研究不同病情严重程度的2019冠状病毒病（COVID-19）患者对SARS-CoV -2的IgM和IgG反应。方法采用化学发光免疫法检测338例新冠肺炎患者的IgM和IgG抗体水平。结果IgM水平在SARS-CoV-2感染后第1周呈升高趋势，2周达到高峰后降至接近背景水平。IgG在1周后检测到，并长期维持在高水平。IgM和/或IgG抗体检测阳性率在轻、重、危重病组间差异无统计学意义。重症和危重症患者IgM水平高于轻症患者，而危重症患者IgG水平低于轻症和重症患者。这可能是由于疾病活动性高和/或危重病例免疫反应受损所致。死亡患者的IgM抗体水平略高于康复患者，但两组患者的IgG水平无显著差异。抗体的纵向检测显示，康复患者的IgM水平迅速下降，而在死亡病例中，IgM水平仍然很高，或者在疾病过程中无法检测到IgM和IgG。结论定量检测抗SARS-CoV-2的IgM和IgG抗体对评估COVID-19的严重程度和预后具有潜在意义。
Objectives This study aimed to determine the IgM and IgG responses against severe acute respiratory syndrome coronavirus (SARS-CoV)-2 in coronavirus disease 2019 (COVID-19) patients with varying illness severities.Methods IgM and IgG antibody levels were assessed via chemiluminescence immunoassay in 338 COVID-19 patients.Results IgM levels increased during the first week after SARS-CoV-2 infection, peaked 2 weeks and then reduced to near-background levels in most patients. IgG was detectable after 1 week and was maintained at a high level for a long period. The positive rates of IgM and/or IgG antibody detections were not significantly different among the mild, severe and critical disease groups. Severe and critical cases had higher IgM levels than mild cases, whereas the IgG level in critical cases was lower than those in both mild and severe cases. This might be because of the high disease activity and/or a compromised immune response in critical cases. The IgM antibody levels were slightly higher in deceased patients than recovered patients, but IgG levels in these groups did not significantly differ. A longitudinal detection of antibodies revealed that IgM levels decreased rapidly in recovered patients, whereas in deceased cases, either IgM levels remained high or both IgM and IgG were undetectable during the disease course.Conclusion Quantitative detection of IgM and IgG antibodies against SARS-CoV-2 quantitatively has potential significance for evaluating the severity and prognosis of COVID-19.