SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.
SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.
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SKAP2通过与小鼠卵母细胞中的WAVE2相互作用来调节Arp2/3复合物以实现肌动蛋白介导的不对称胞质分裂
DOI：
10.1080/15384101.2017.1380126
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wang HL
中科院分区：
文献类型：
--
作者：
He SW;Xu BH;Liu Y;Wang YL;Chen MH;Xu L;Liao BQ;Lui R;Li FP;Lin YH;Fu XP;Fu BB;Hong ZW;Liu YX;Qi ZQ;Wang HL
SKAP 2（Src激酶相关磷蛋白2），Src家族激酶的底物，已被认为参与肌动蛋白介导的细胞过程。然而，关于其在小鼠卵母细胞成熟中的作用知之甚少。因此，在这项研究中，我们研究SKAP 2在小鼠卵母细胞不对称分裂过程中的表达，定位和功能。SKAP 2蛋白在小鼠卵母细胞的各个发育阶段均有表达。免疫荧光染色显示，SKAP 2主要分布在卵母细胞成熟过程中的皮质。用细胞松弛素B处理卵母细胞证实SKAP 2与肌动蛋白共定位。通过注射特异性短干扰RNA耗尽SKAP 2导致纺锤体迁移失败、极体挤出和胞质分裂缺陷。同时，这些处理后，在卵母细胞膜和细胞质中的肌动蛋白丝的染色显着减少。SKAP 2的耗竭也破坏了肌动蛋白帽和皮质颗粒自由域的形成，并在末期阶段逮捕了大比例的卵母细胞。SKAP 2活性降低后，Arp 2/3复合物和WAVE 2表达降低。我们的研究结果表明，SKAP 2调节Arp 2/3复合物，并通过与WAVE 2在小鼠卵母细胞中相互作用，肌动蛋白介导的不对称胞质分裂是必不可少的。
SKAP2 (Src kinase-associated phosphoprotein 2), a substrate of Src family kinases, has been suggested to be involved in actin-mediated cellular processes. However, little is known about its role in mouse oocyte maturation. In this study, we thus investigated the expression, localization, and functions of SKAP2 during mouse oocyte asymmetric division. SKAP2 protein expression was detected at all developmental stages in mouse oocytes. Immunofluorescent staining showed that SKAP2 was mainly distributed at the cortex of the oocytes during maturation. Treatment with cytochalasin B in oocytes confirmed that SKAP2 was co-localized with actin. Depletion of SKAP2 by injection with specific short interfering RNA caused failure of spindle migration, polar body extrusion, and cytokinesis defects. Meanwhile, the staining of actin filaments at the oocyte membrane and in the cytoplasm was significantly reduced after these treatments. SKAP2 depletion also disrupted actin cap and cortical granule-free domain formation, and arrested a large proportion of oocytes at the telophase stage. Moreover, Arp2/3 complex and WAVE2 expression was decreased after the depletion of SKAP2 activity. Our results indicate that SKAP2 regulates the Arp2/3 complex and is essential for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.
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影响因子：
5.5
作者：
Kutsuna, H;Suzuki, K;Kitagawa, S
通讯作者：
Kitagawa, S
影响因子：
2.7
作者：
LONGO, FJ;CHEN, DY
通讯作者：
CHEN, DY
DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
21.3
作者：
Leader, B;Lim, H;Leder, P
通讯作者：
Leder, P
影响因子：
9.2
作者：
Azoury, Jessica;Lee, Karen W.;Verlhac, Marie-Helene
通讯作者：
Verlhac, Marie-Helene