Melanopsin elevates locomotor activity during the wake state of the diurnal zebrafish.
Melanopsin elevates locomotor activity during the wake state of the diurnal zebrafish.
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黑视蛋白可提高昼间斑马鱼清醒状态下的运动活动。
DOI：
10.15252/embr.202051528
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发表时间：
2022-05-04
期刊：
影响因子：
7.7
通讯作者：
中科院分区：
文献类型：
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作者：
哺乳动物和鱼类的松果体通过释放褪黑激素，在使行为适应环境光条件方面起着关键作用。在小鼠中，光通过非视觉光感受器黑视蛋白抑制夜间运动活动。与广泛研究的黑视素在啮齿动物松果体间接调节中的功能相反，其在固有光敏性斑马鱼松果体中的作用尚未阐明。因此，光信号机制在远距离脊椎动物之间是否保守以及黑视素如何影响昼夜行为并不明显。在昼夜活动的斑马鱼中产生了黑视蛋白（opn4.1-opn4xb）的双重敲除，这表现出清醒状态下运动活动减弱。转录组测序深入了解了黑视素下游的途径，这意味着褪黑激素途径的持续抑制是在昼夜清醒状态下提高运动活性所必需的。此外，我们表明，光诱导运动活动在昼夜唤醒状态在强度依赖性的方式。这些观察结果表明，斑马鱼和哺乳动物之间存在一种共同的黑视素驱动机制，而昼夜节律和夜间节律在褪黑激素下游受到反向调节。这项研究提出了在斑马鱼昼夜唤醒状态下检测到的光子数和运动活动之间的相关性。光通过黑视素介导的褪黑激素通路抑制来提高活性水平。
Mammalian and fish pineals play a key role in adapting behaviour to the ambient light conditions through the release of melatonin. In mice, light inhibits nocturnal locomotor activity via the non‐visual photoreceptor Melanopsin. In contrast to the extensively studied function of Melanopsin in the indirect regulation of the rodent pineal, its role in the intrinsically photosensitive zebrafish pineal has not been elucidated. Therefore, it is not evident if the light signalling mechanism is conserved between distant vertebrates, and how Melanopsin could affect diurnal behaviour. A double knockout of melanopsins (opn4.1‐opn4xb) was generated in the diurnal zebrafish, which manifests attenuated locomotor activity during the wake state. Transcriptome sequencing gave insight into pathways downstream of Melanopsin, implying that sustained repression of the melatonin pathway is required to elevate locomotor activity during the diurnal wake state. Moreover, we show that light induces locomotor activity during the diurnal wake state in an intensity‐dependent manner. These observations suggest a common Melanopsin‐driven mechanism between zebrafish and mammals, while the diurnal and nocturnal chronotypes are inversely regulated downstream of melatonin. This study proposes a correlation between the number of detected photons and locomotor activity in the diurnal wake state in zebrafish. Light elevates the activity level through a Melanopsin‐mediated repression of the melatonin pathway.
影响因子：
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作者：
Rohde K;Møller M;Rath MF
通讯作者：
Rath MF