KazrinA is required for axial elongation and epidermal integrity in Xenopus tropicalis.
KazrinA is required for axial elongation and epidermal integrity in Xenopus tropicalis.
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KazrinA 是热带爪蟾轴向伸长和表皮完整性所必需的。
DOI：
10.1002/dvdy.21557
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Smith,JamesC
中科院分区：
文献类型：
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作者：
Sevilla,LisaM;Rana,AmerA;Watt,FionaM;Smith,JamesC
Kazrin 是最近描述的一种桥粒蛋白，可结合角化包膜前体周斑蛋白。在这项研究中，我们检查了热带爪蟾发育过程中的 kazrinisoformA 表达，并研究了其缺失的后果。整体原位杂交显示，kazrinAmRNA 在整个胚胎中表达，至少直到蝌蚪阶段。注射了针对 kazrinA 的反义吗啉寡核苷酸的热带非洲爪蟾胚胎无法正常伸长，并且在头部、眼睛、脊索和体节发育中表现出缺陷。我们还观察到表皮变得杂乱并经常与下面的中胚层分离，导致表皮水疱的形成。总之，我们的结果表明 kazrinA 的缺失会导致细胞粘附缺陷，从而影响轴向伸长、细胞分化和表皮形态发生。发育动力学 237:1718‐1725, 2008。© 2008 Wiley‐Liss, Inc.
Kazrin is a recently described desmosomal protein that binds the cornified envelope precursor periplakin. In this study, we have examinedkazrinisoformAexpression during the development ofXenopus tropicalisand investigated the consequences of its depletion. Whole mount in situ hybridisation revealed thatkazrinAmRNA is expressed throughout the embryo at least until tadpole stages.Xenopus tropicalisembryos that had been injected with antisense morpholino oligonucleotides directed against kazrinA failed to elongate properly and showed defects in development of the head, eye, notochord, and somites. We also observed that the epidermis became disorganised and frequently separated from the underlying mesoderm, causing the formation of epidermal blisters. Together, our results suggest that loss of kazrinA causes defects in cell adhesion that affect axial elongation, cell differentiation, and epidermal morphogenesis. Developmental Dynamics 237:1718‐1725, 2008. © 2008 Wiley‐Liss, Inc.