Combined action of interferons and transforming growth factor beta on the proliferation of human fibroblasts.
Combined action of interferons and transforming growth factor beta on the proliferation of human fibroblasts.
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干扰素和转化生长因子β对人成纤维细胞增殖的联合作用。
DOI：
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发表时间：
1991
影响因子：
3.2
通讯作者：
A. Inglot
中科院分区：
文献类型：
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作者：
W. Popik;A. Inglot
研究了干扰素（IFNs）和转化生长因子- β (tgf - β)单独和联合使用对人胚胎二倍体成纤维细胞增殖的影响。实验条件标准化，培养基中含有2%的单批次胎牛血清，这几乎是细胞附着和生长所需的最低剂量。观察到的效应与剂量有关。浓度为1至100 U/ml的ifn （α、β或γ型）或浓度为0.1至1.0 ng/ml的tgf - β，与不含这些因素的对照培养物相比，能刺激细胞增殖20%至25%。在较高剂量下，IFNs(10（2)至10(5)U/ml）或tgf - β（1至10 ng/ml）均能抑制细胞的增殖。ifn与tgf - β具有协同作用。我们认为ifn和tgf - β是双功能的生长调节剂，尽管它们的抗有丝分裂作用比它们可能的生长刺激活性更重要。后一种作用可能是由于ifn或tgf - β与细胞培养中存在的其他因子相互作用而产生的次要现象。我们已经表明，表皮生长因子（EGF）可能增加低剂量的ifn - γ或tgf - β的生长刺激作用。
The effects of interferons (IFNs) and transforming growth factor-beta (TGF-beta) used alone and in combination on the multiplication of human embryonic diploid fibroblasts were studied. The experimental conditions were standardized and medium containing 2% of a single batch of fetal calf serum, which was almost minimal dose required for good the cell attachment and growth, was used. The observed effects were dose related. IFNs (types alpha, beta, or gamma) at concentration of 1 to 100 U/ml or TGF-beta at concentration of 0.1 to 1.0 ng/ml stimulated the cell multiplication by 20 to 25% when compared to the control cultures incubated without the factors. At higher doses both IFNs (10(2) to 10(5) U/ml) or TGF-beta (1 to 10 ng/ml) inhibited the proliferation of the cells. The antimitogenic action of IFNs and TGF-beta was synergistic. We suggest that IFNs as well as TGF-beta are bifunctional growth regulators although their antimitotic action dominates over their possible growth stimulatory activity. The latter action may be a secondary phenomenon due to interaction of IFNs or TGF-beta with other factors present in cell culture. We have shown that epidermal growth factor (EGF) may increase the growth stimulatory action of low doses of IFN-gamma or TGF-beta.