Renal expression of IL-6 and TNFalpha genes in lupus nephritis.
Renal expression of IL-6 and TNFalpha genes in lupus nephritis.
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DOI：
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发表时间：
1998
期刊：
影响因子：
2.6
通讯作者：
R. Herrera-Esparza;O. Barbosa-Cisneros;R. Villalobos-Hurtado;E. Avalos-Díaz
中科院分区：
文献类型：
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作者：
R. Herrera-Esparza;O. Barbosa-Cisneros;R. Villalobos-Hurtado;E. Avalos-Díaz
进行这些研究以检查狼疮性肾炎患者肾脏中炎性细胞因子IL-6和TNF α的存在，作为它们在其发病机制中可能作用的指标。采用单克隆抗IL-6和TNF α抗体，通过直接免疫荧光法对19例III型或IV型狼疮性肾炎患者的肾活检进行处理。这些基因的局部表达被证明是通过原位杂交和从肾组织中分离的总RNA的逆转录-PCR扩增。52%的活检显示IL-6和TNF α沿着肾小球和肾小管沉积;在6例IV型和1例III型肾炎活检中证实了这些细胞因子的原位表达。炎症细胞因子在狼疮性肾炎患者的肾脏中活跃合成，因此可能在其发病机制中发挥作用。
These studies were carried out to examine the presence of the inflammatory cytokines IL-6 and TNFalpha in kidneys of patients with lupus nephritis as an indicator of their possible role in its pathogenesis. A total of 19 kidney biopsies from patients with type III or IV lupus nephritis were processed by direct immunofluorescence using monoclonal anti-IL-6 and TNFalpha antibodies. Local expression of these genes was demonstrated both by in situ hybridization and by reverse transcriptase-PCR amplification of total RNA isolated from kidney tissue. Fifty-two percent of the biopsies exhibited IL-6 and TNFalpha deposited along the glomeruli and tubules; in situ expression of these cytokines was demonstrated in 6 biopsies with type IV, and 1 with type III nephritis. Inflammatory cytokines are actively synthesized in the kidneys of patients with lupus nephritis and therefore, may play a role in its pathogenesis.