Shenfu injection attenuates neurotoxicity of bupivacaine in cultured mouse spinal cord neurons.
Shenfu injection attenuates neurotoxicity of bupivacaine in cultured mouse spinal cord neurons.
复制标题
DOI：
10.1097/00029330-200711020-00004
复制
发表时间：
2007-11
影响因子：
6.1
通讯作者：
L. Xiong;Qiang Wang;Mu-yun Liu;Ye Peng;Qing-bo Li;Zhi-hong Lu;C. Lei
中科院分区：
文献类型：
--
作者：
L. Xiong;Qiang Wang;Mu-yun Liu;Ye Peng;Qing-bo Li;Zhi-hong Lu;C. Lei
背景：我们先前在大鼠体内的研究表明，参附注射液是一种临床上常用的中药提取物制剂，可以减轻局部麻醉剂布比卡因静脉滴注所致的神经和心脏毒性。本研究旨在探讨布比卡因对原代培养的小鼠脊髓神经元是否具有毒性作用，以及参附注射液在细胞模型中是否具有类似的神经保护作用。方法取11~14日龄胎鼠脊髓，切碎后孵育。在培养液中加入阿糖胞苷抑制非神经细胞的增殖。用β-微管蛋白免疫细胞化学染色确定培养细胞的特性。将培养的神经细胞随机分为3组，分别给予不同剂量的布比卡因、参附和布比卡因+参附作用48h。用四甲基偶氮唑蓝(四甲基偶氮唑蓝)比色法检测细胞存活率。结果0.01%、0.02%、0.04%和0.08%浓度的布比卡因可剂量依赖性地降低培养神经元的存活率。参附注射液在1/50~1/12.5(V/V)浓度范围内对培养神经元的存活率无明显影响(P<；0.05与对照组相比)，但对0.03%布比卡因预处理的神经元存活率有显著的促进作用(P<；0.05)。结论参附注射液本身对脊髓神经元无影响，但能减轻布比卡因所致的神经毒性。
BACKGROUND Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in primary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model. METHODS The spinal cords from 11- to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of beta-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine + Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium (MTT) assay. RESULTS The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 (V/V) had no significant influence on the viability of cultured neurons (P < 0.05 vs control), the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine (P < 0.05). CONCLUSION Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaine-induced neurotoxicity in vitro.