Quinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater
Quinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater
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DOI：
10.1016/j.scitotenv.2015.10.124
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发表时间：
2016-01-15
影响因子：
9.8
通讯作者：
Manaia, Celia M.
中科院分区：
文献类型：
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作者：
Varela, Ana Rita;Nunes, Olga C.;Manaia, Celia M.
气单胞菌属的成员被认为是水生环境中抗生素耐药性的携带者。然而，人们对它们对于从医院废水向环境传播耐药性的重要性却知之甚少。喹诺酮类耐药气单胞菌属(n = 112) 从医院废水 (HE) 以及接收城市污水处理厂 (UWTP) 的原废水 (RWW) 和处理废水 (TWW) 中分离出来的物质进行了表征。基于 16S rRNA、cpn60 和 gyrB 基因序列分析评估物种鉴定和种内遗传多样性。根据起源和分类群的功能分析抗生素耐药性表型和基因型（qnrA、qnrB、qnrC、qnrD、qnrS、qnrVC；qepA；oqxAB；aac(6')-Ib-cr；bla(OXA)；incU）。大多数分离株属于豚鼠气单胞菌和嗜水气单胞菌（分别为 50% 和 41%）。喹诺酮类和β-内酰胺酶抗性基因 aac(6')-Ib-cr 和 bla(OXA)，包括首次在气单胞菌属中鉴定的基因 bla(OXA)-101，分别在 58% 和 56% 的分离株中检测到，在 HE 和 UWTP 废水中的患病率相同。相比之下，基因 qnrS2 主要在 UWTP 分离株 (51%) 中观察到，而在 HE 分离株 (3%) 中很少观察到，这表明其起源不是临床环境。能够识别抗生素耐药性传播主要途径的细菌群和基因是控制这一问题的宝贵工具。在这项研究中，得出的结论是，携带基因 aac(6')-Ib-cr 和 blaOXA 的气单胞菌属成员是废水生境中抗生素耐药性传播的相关示踪剂，而产生基因 qnrS2 的气单胞菌属成员则可以从非临床来源进行追踪。 (C) 2015 Elsevier B.V. 保留所有权利。
Members of the genus Aeromonas are recognized carriers of antibiotic resistance in aquatic environments. However, their importance on the spread of resistance from hospital effluents to the environment is poorly understood. Quinolone resistant Aeromonas spp. (n = 112) isolated from hospital effluent (HE) and from raw (RWW) and treated wastewater (TWW) of the receiving urban wastewater treatment plant (UWTP) were characterized. Species identification and genetic intraspecies diversity were assessed based on the 16S rRNA, cpn60 and gyrB genes sequence analysis. The antibiotic resistance phenotypes and genotypes (qnrA, qnrB, qnrC, qnrD, qnrS, qnrVC; qepA; oqxAB; aac(6')-Ib-cr; bla(OXA); incU) were analyzed in function of the origin and taxonomic group. Most isolates belonged to the species Aeromonas caviae and Aeromonas hydrophila (50% and 41%, respectively). The quinolone and the beta-lactamase resistance genes aac(6')-Ib-cr and bla(OXA), including gene bla(OXA)-101, identified for the first time in Aeromonas spp., were detected in 58% and 56% of the isolates, respectively, with identical prevalence in HE and UWTP wastewater. In contrast, the gene qnrS2 was observed mainly in isolates from the UWTP (51%) and rarely in HE isolates (3%), suggesting that its origin is not the clinical setting.Bacterial groups and genes that allow the identification of major routes of antibiotic resistance dissemination are valuable tools to control this problem. In this study, it was concluded that members of the genus Aeromonas harboring the genes aac(6')-Ib-cr and blaOXA are relevant tracers of antibiotic resistance dissemination in wastewater habitats, while those yielding the gene qnrS2 allow the traceability from non-clinical sources. (C) 2015 Elsevier B.V. All rights reserved.