Altered expression of progesterone receptors in testis of infertile men
Altered expression of progesterone receptors in testis of infertile men
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DOI：
10.1016/s1472-6483(10)60192-7
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发表时间：
2008-08-01
影响因子：
4
通讯作者：
Modi, Deepak
中科院分区：
文献类型：
--
作者：
Abid, Shadaan;Gokral, Jyotsna;Modi, Deepak
黄体酮与精子发生过程有关。本研究旨在探讨不育男性睾丸中孕激素受体（PR）表达与精子发生的关系。采用原位杂交和uninino组织化学方法对18例不育男性睾丸活检组织的PR rnRNA和蛋白进行了检测。采用Johnsen评分法评估精子发生的程度。本研究中所有患者均无Ycl基因微缺失。在所有仅显示支持细胞（SCO）的睾丸切片或在精索细胞处阻滞的睾丸切片中几乎检测不到PR表达。细胞质表达弱，在减数分裂的不同阶段表现为阻滞。在精原期至圆形精原期的活检中，不同类型细胞的细胞核和胞质中均可见PR 0 n的表达，且表达强度低于正常活检。活组织检查显示精子发育不足，PR表达正常。在表现为非固定表型的活组织检查中，SCO或膈肌收缩停止的小管没有PR表达；邻近小管PR表达正常，显示精子生成完全。不同表型的不育男性睾丸活检中PR表达和Johnsen评分的血清定量评估表明PR表达与精子发生程度有直接关系。
Progesterone has been implicated in the process of spermatogenesis. This study airned to investigate the association of progesterone receptor (PR) expression with spermatogenesis in the testis of infertile men. PR rnRNA and protein were assessed by in-situ hybridization and unInLinohistochernistry in testicular biopsies obtained frorn 18 infertile inen. The extent of sperniatogenesis was assessed by Johnsen scoring. None of the patients included in the Study had Ycl alicrodeletions. PR expression was almost undetcctable in all the testicular sections displaying Sertoli cell only (SCO) or arrest at sperniatogonia. Weak cytoplasmic expression was observed in biopsies showing arrest at different stages of meiosis. In biopsies displaying sperinatogencsis up to the round sperinalid stages, PR expressi 0 n was observed in both nucleus and cytoplasin of different cell types at intensity lower than that detected in normal biopsies. Normal PR expression was observed in biopsies demonstrating hyposperniatogenesis. In biopsies showing illixed phenotypes, the tubules with SCO or spenuatopnia arrest showed absence of PR expression; normal PR expression was observed in adjacent tubules showing complete sperniatooenesis. Serni-cluantitative assessrnen of PR expression and Johnsen scores in the testicular biopsies of infertile inen dernonstratinIg different phenotypes indicated a direct relationship between PR expression and extent of spermatogenesis.