Angiogenesis assays: A critical overview
Angiogenesis assays: A critical overview
复制标题
DOI：
10.1373/49.1.32
复制
发表时间：
2003-01-01
影响因子：
9.3
通讯作者：
Akhtar, N
中科院分区：
文献类型：
--
作者：
Auerbach, R;Lewis, R;Akhtar, N
背景：血管生成，即新血管的形成，是正常发育过程和许多病理学的一个组成部分，从肿瘤生长和转移到炎症和眼部疾病。血管生成测定用于测试促血管生成剂和抗血管生成剂的功效。方法：大多数血管生成诱导剂和抑制剂的研究依赖于体外和体内的各种模型作为功效指标。在本报告中，我们描述了目前使用的主要方法：体内基质胶塞和角膜新生血管形成测定、体内/体外鸡绒毛尿囊膜（CAM）测定、以及体外细胞（增殖、迁移、管形成）和器官型（主动脉环）测定。我们包括对两种新方法的描述，即小鸡主动脉弓和基质胶海绵测定。结论：体外测试很有价值，可以迅速进行，并且适合量化，但必须极其谨慎地解释。体外测试最好被视为提供初步信息，并通过体内测定进行确认。应使用多项测试以获得体外测试的最大益处。体内测试执行起来更加困难和耗时，从而限制了可以同时运行的测试数量。量化通常也更加困难。然而，体内测定是必不可少的，因为血管对测试试剂的反应性质复杂，没有体外模型可以完全实现这种反应。 (C) 2003 年美国临床化学协会。
Background: Angiogenesis, the formation of new blood vessels, is an integral part of both normal developmental processes and numerous pathologies, ranging from tumor growth and metastasis to inflammation and ocular disease. Angiogenesis assays are used to test efficacy of both pro- and antiangiogenic agents.Methods: Most studies of angiogenesis inducers and inhibitors rely on various models, both in vitro and in vivo, as indicators of efficacy. In this report we describe the principal methods now in use: the in vivo Matrigel plug and corneal neovascularization assays, the in vivo/in vitro chick chorioallantoic membrane (CAM) assay, and the in vitro cellular (proliferation, migration, tube formation) and organotypic (aortic ring) assays. We include description of two new methods, the chick aortic arch and the Matrigel sponge assays.Conclusions: In vitro tests are valuable, can be carried out expeditiously, and lend themselves to quantification, but must be interpreted with extreme caution. In vitro tests are best viewed as providing initial information, subject to confirmation by in vivo assays. Multiple tests should be used to obtain maximum benefit from in vitro tests. In vivo tests are more difficult and time-consuming to perform, thereby limiting the number of tests that can run at any one time. Quantification is generally more difficult as well. However, in vivo assays are essential because of the complex nature of vascular responses to test reagents, responses that no in vitro model can fully achieve. (C) 2003 American Association for Clinical Chemistry.