Spatiotemporal transcriptome provides insights into early fruit development of tomato (Solanum lycopersicum).
Spatiotemporal transcriptome provides insights into early fruit development of tomato (Solanum lycopersicum).
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时空转录组提供了对番茄（Solanum lycopersicum）早期果实发育的见解
DOI：
10.1038/srep23173
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发表时间：
2016-03-18
影响因子：
4.6
通讯作者：
Cui X
中科院分区：
文献类型：
--
作者：
Zhang S;Xu M;Qiu Z;Wang K;Du Y;Gu L;Cui X
番茄果实早期发育对作物生产至关重要。受精后，卵巢在成熟前经历细胞分裂和细胞扩张。虽然激素和碳水化合物等调控信号在果实早期发育中的作用已经被研究，但这些调控信号的空间分布和顺序启动仍然需要探索。以番茄品种‘Moneymeker’为材料，利用体视镜对番茄早期果实四个不同发育阶段的胚珠和子房壁/果皮的转录组进行了分析。这些数据集为我们提供了胚珠和果皮早期发育过程中信号的时空分布的全貌。我们的结果表明，受精后，激素信号在胚珠和果皮中都被启动了。之后，由于胚珠和果皮发育过程的不同，不同的信号在胚珠和果皮中激活。我们的研究为基因表达的时空调控提供了前人没有研究过的序列信息，并进一步加强了对控制果实早期发育的调控和代谢事件的理解。
Early fruit development is crucial for crop production in tomato. After fertilization, the ovary undergoes cell division and cell expansion before maturation. Although the roles of regulatory signals such as hormone and carbohydrate during early fruit development have been studied, the spatial distribution and the sequential initiation of these regulatory signals still need to be explored. Using the tomato cultivar ‘Moneymaker’, we analyzed the transcriptome of the ovule and the ovary wall/pericarp dissected from four different stages of the early developing fruits by stereoscope. These datasets give us the whole picture about the spatial and temporal signal distribution in early development of ovule and pericarp. Our results indicate that the hormone signal was initiated in both ovule and pericarp after fertilization. After that, different signals were activated in ovule and pericarp due to their distinct developmental processes. Our study provides spatiotemporal regulatory landscape of gene expression with sequential information which was not studied by previous work and further strengthens the comprehension of the regulatory and metabolic events controlling early fruit development.