Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
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DOI：
10.1099/mic.0.055939-0
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发表时间：
2012-03-01
期刊：
影响因子：
2.8
通讯作者：
Dymock, David
中科院分区：
文献类型：
--
作者：
Cogoni, Valentina;Morgan-Smith, Alex;Dymock, David
齿密螺旋体广泛存在于人类口腔中，主要与牙周病的发生发展有关的细菌群落有关。整合到生物膜群落中的能力对口腔细菌的生长和生存至关重要，涉及细菌间的共聚、代谢合作和针对宿主防御的协同作用。在这篇文章中，我们发现在细胞表面的高分子复合体中发现的类糜蛋白酶(CTLP)，介导I齿状菌与其他潜在的牙周病原体，如牙龈卟啉单胞菌、核梭杆菌、中间普里沃特氏菌和微小细小单胞菌的黏附。相互作用本身似乎不需要蛋白分解活性，除了结合Parv外，主要的外鞘蛋白(MSP)也不是必需的。迈克拉。在唾液膜上形成了密集的细胞和基质生物膜，最深可达40 pm，齿状毛滴虫和牙龈假单胞菌之间形成生物膜，上层细胞丰富。CTLP的表达，而不是MSP的表达，对牙龈假单胞菌的双物种生物膜的形成至关重要。在不同条件下，牙周炎杆菌不能与其他三种牙周细菌中的任何一种形成双物种生物膜。齿状毛滴虫和牙龈假单胞菌之间的协同作用还表现为增加了对凝血的抑制，这是CTLP依赖的。这些结果证明了CTLP在齿状毛滴虫与其他口腔微生物相互作用中的关键作用，导致了微生物群落发育和宿主组织致病的协同作用。
Treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. The ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. In this article we show that the chymotrypsin-like proteinase (CTLP), found within a high-molecular-mass complex on the cell surface, mediates adherence of I denticola to other potential periodontal pathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia and Parvimonas micra. Proteolytic activity per se did not appear to be required for the interactions, and expression of the major outer-sheath protein (Msp) was not necessary, except for binding Parv. micra. Biofilms of densely packed cells and matrix, up to 40 pm in depth, were formed between T. denticola and P. gingivalis on salivary pellicle, with T. denticola cells enriched in the upper layers. Expression of CTLP, but not Msp, was critical for dual-species biofilm formation with P. gingivalis. T. denticola did not form dual-species biofilms with any of the other three periodontal bacterial species under various conditions. Synergy between T. denticola and P. gingivalis was also shown by increased inhibition of blood clotting, which was CTLP-dependent. The results demonstrate the critical role of CTLP in interactions of T. denticola with other oral micro-organisms, leading to synergy in microbial community development and host tissue pathogenesis.