Comparative Transcriptome Analysis Reveals the Potential Cardiovascular Protective Targets of the Thyroid Hormone Metabolite 3-Iodothyronamine (3-T1AM)
Comparative Transcriptome Analysis Reveals the Potential Cardiovascular Protective Targets of the Thyroid Hormone Metabolite 3-Iodothyronamine (3-T1AM)
复制标题
比较转录组分析揭示了甲状腺激素代谢物 3-碘甲腺胺 (3-T1AM) 的潜在心血管保护靶点
DOI：
10.1155/2020/1302453
复制
发表时间：
2020-06
影响因子：
--
通讯作者：
Xingde Liu
中科院分区：
文献类型：
--
作者：
Haiyan Zhou;Bailong Hu;Bei Zhang;Yiming Wang;Xingde Liu
甲状腺激素代谢物3-碘甲状腺胺（3-T1AM）在降低心率和心输出量方面正迅速成为一种有前景的化合物。我们的研究目的是全面了解3-T1AM对心肌细胞的分子机制及其在心血管疾病中的潜在靶点。材料与方法在我们的研究中，我们利用RNA-Seq来表征3-T1AM处理后H9C2细胞的基因表达。通过比较转录组分析，包括基因本体、信号通路、疾病连通性分析和蛋白-蛋白相互作用网络（PPI），发现关键基因功能、枢纽基因和相关通路。结果在3-T1AM处理的H9C2细胞中共鉴定出1494个差异表达基因（deg），其中上调基因192个，下调基因1302个。其中，90个基因与心血管疾病有关。PPI分析表明，5个枢纽基因可能是3-T1AM的靶点。随后，通过RT-qPCR分析证实了8个用RNA-Seq鉴定的deg。结论本研究全面分析了3-T1AM对H9C2细胞的影响，为3-T1AM对心血管疾病的治疗干预提供了新的思路。
Background The thyroid hormone metabolite 3-iodothyronamine (3-T1AM) is rapidly emerging as a promising compound in decreasing the heart rate and lowering the cardiac output. The aim of our study was to fully understand the molecular mechanism of 3-T1AM on cardiomyocytes and its potential targets in cardiovascular diseases. Materials and Methods In our study, we utilized RNA-Seq to characterize the gene expression in H9C2 cells after 3-T1AM treatment. Comparative transcriptome analysis, including gene ontology, signaling pathways, disease connectivity analysis, and protein-protein interaction networks (PPI), was presented to find the critical gene function, hub genes, and related pathways. Results A total of 1494 differently expressed genes (DEGs) were identified (192 upregulated and 1302 downregulated genes) in H9C2 cells for 3-T1AM treatment. Of these, 90 genes were associated with cardiovascular diseases. The PPI analysis indicated that 5 hub genes might be the targets of 3-T1AM. Subsequently, eight DEGs characterized using RNA-Seq were confirmed by RT-qPCR assays. Conclusions Our study provides a comprehensive analysis of 3-T1AM on H9C2 cells and delineates a new insight into the therapeutic intervention of 3-T1AM for the cardiovascular diseases.
登录
查看更多内容
HMGB1 通过 RAGE/ERK1/2 信号通路增强体外机械应力诱导的心肌细胞肥大
影响因子：
5.4
作者：
Zhang Lei;Yang Xue;Jiang Guoliang;Yu Ying;Wu Han;Su Yangang;Sun Aijun;Zou Yunzeng;Jiang Hong;Ge Junbo
通讯作者：
Ge Junbo
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
通过微阵列和生物信息学分析比较T-2毒素和脱氧雪腐镰刀菌烯醇对人软骨细胞的毒性机制
影响因子：
3.5
作者：
Lei Yang;Suiqin Wang;Guanghui Zhao;Xi Wang;Xiong Guo
通讯作者：
Xiong Guo
影响因子：
5.6
作者：
Rogowski, Michael;Bellusci, Lorenza;Assadi-Porter, Fariba M.
通讯作者：
Assadi-Porter, Fariba M.
影响因子：
48
作者：
Mortazavi, Ali;Williams, Brian A.;Wold, Barbara
通讯作者：
Wold, Barbara