Cross-reacting xenoantibodies to the heavy chain of H-2 and HLA-A,B antigens: serologic and immunochemical characterization.
Cross-reacting xenoantibodies to the heavy chain of H-2 and HLA-A,B antigens: serologic and immunochemical characterization.
复制标题
异种抗体与 H-2 和 HLA-A、B 抗原重链的交叉反应：血清学和免疫化学表征。
DOI：
10.1111/j.1365-3083.1980.tb00070.x
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发表时间：
1980
影响因子：
3.7
通讯作者：
Ferrone,S
中科院分区：
文献类型：
--
作者：
Callahan,GN;Pellegrino,MA;Ferrone,S
H‐2 cross‐reactive antibodies present in HLA xenoantisera were purified by absorption on and elution from murine cells. Antibodies isolated from one of these antisera. 78‐E48, were found to mediate complement‐dependent lysis of both human and murine lymphoid cells but not of Daudi cells or of human lymphoid cells coaled with Fab2fragments from a cow anti‐human β2‐microglobulin (β2m) antiserum. In indirect immunoprecipitation analyses 78‐E48 reacted only with proteins of approximately 45,000 and 12,000 MW present in NP‐40 extracts of both human and murine lymphoid cells. Sequential precipitation experiments with rabbit anti‐human β2m and allo‐anti‐H‐2Kk, Iaksera established that these proteins were in fact H‐2 and HLA‐A, B antigens. It was also found that 78‐E48 reacted only with the heavy chain of HLA‐A, B and H‐2 antigens, since this eluate was unreactive with β2m in a radioimmunoassay, and its capacity to immunoprecipitate the 45,000 and 12,000 MW proteins from human cell extracts was unaffected by prior reaction with purified human β2m. These data show for the first time that H‐2 and HLA‐A, B antigens share properties that probably depend upon their tertiary structure.
