Evaluation of three methods for retrospective correction of vignetting on medical microscopy images utilizing two open source software tools
Evaluation of three methods for retrospective correction of vignetting on medical microscopy images utilizing two open source software tools
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DOI：
10.1111/j.1365-2818.2011.03546.x
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Perrea, Despoina
中科院分区：
文献类型：
--
作者：
Babaloukas, Georgios;Tentolouris, Nicholas;Perrea, Despoina
在应用图像分析之前，对安装在显微镜上的数码相机获得的图像进行晕晕校正是必不可少的。本研究的目的是评估三种医学显微镜图像晕影的回溯性校正方法，并将它们与前瞻性校正方法进行比较。使用了来自四个不同组织的一张数字图像，并在每一张图像上应用了晕影效果。产生的晕影图像被复制了四次，在每个复制品中，斐济和GIMP软件工具采用了不同的晕影校正方法。从每种方法与原始图像的比较中，所有组织中的预期方法都获得了最高的峰值信噪比。形态滤波法是回溯性方法中峰值信噪比最高的方法。建议将前瞻性方法作为晕晕校正的首选方法，如果不适用，则可以建议采用形态滤波作为回溯性替代方法。
Correction of vignetting on images obtained by a digital camera mounted on a microscope is essential before applying image analysis. The aim of this study is to evaluate three methods for retrospective correction of vignetting on medical microscopy images and compare them with a prospective correction method. One digital image from four different tissues was used and a vignetting effect was applied on each of these images. The resulted vignetted image was replicated four times and in each replica a different method for vignetting correction was applied with fiji and gimp software tools. The highest peak signal-to-noise ratio from the comparison of each method to the original image was obtained from the prospective method in all tissues. The morphological filtering method provided the highest peak signal-to-noise ratio value amongst the retrospective methods. The prospective method is suggested as the method of choice for correction of vignetting and if it is not applicable, then the morphological filtering may be suggested as the retrospective alternative method.