Identification of multipotent stem cells from adult dog periodontal ligament.
Identification of multipotent stem cells from adult dog periodontal ligament.
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DOI：
10.1111/j.1600-0722.2012.00975.x
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发表时间：
2012-08
影响因子：
1.9
通讯作者：
Wen-jun Wang;Yuming Zhao;B. Lin;Jie Yang;L. Ge
中科院分区：
文献类型：
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作者：
Wen-jun Wang;Yuming Zhao;B. Lin;Jie Yang;L. Ge
牙周病的特点是负责限制颌骨内牙齿的结缔组织的破坏，是牙齿脱落的主要原因。人牙周膜干细胞介导的牙周组织再生可能为牙周病的治疗提供一种新的策略。狗是一种广泛使用的大型动物模型，用于牙周疾病进展，组织再生和牙科植入物的研究，但很少有人注意到该物种中涉及的细胞的鉴定。本研究旨在鉴定从犬牙周膜分离的干细胞（cPDLSCs）。与hPDLSC一样，cPDLSC显示出克隆形成能力，并表达间充质干细胞标志物STRO-1、CD 146和CD 105，但不表达CD 34。诱导成骨后，cPDLSCs在体外显示钙积累。此外，cPDLSCs还显示出成脂和成软骨潜能。与无细胞对照组相比，在cPDLSC移植的CB-17/SCID小鼠中观察到更多的牙骨质/牙周韧带样结构。这些结果表明，cPDLSCs是克隆性的，高度增殖，并具有多向分化潜能，它们可以被用作一种新的细胞治疗方法，以促进成功的和更可预测的牙周组织再生使用犬牙周病模型。
Periodontal diseases, which are characterized by destruction of the connective tissues responsible for restraining the teeth within the jaw, are the main cause of tooth loss. Periodontal regeneration mediated by human periodontal ligament stem cells (hPDLSCs) may offer an alternative strategy for the treatment of periodontal disease. Dogs are a widely used large-animal model for the study of periodontal-disease progression, tissue regeneration, and dental implants, but little attention has been paid to the identification of the cells involved in this species. This study aimed to characterize stem cells isolated from canine periodontal ligament (cPDLSCs). The cPDLSCs, like hPDLSCs, showed clonogenic capability and expressed the mesenchymal stem cell markers STRO-1, CD146, and CD105, but not CD34. After induction of osteogenesis, cPDLSCs showed calcium accumulation in vitro. Moreover, cPDLSCs also showed both adipogenic and chondrogenic potential. Compared with cell-free controls, more cementum/periodontal ligament-like structures were observed in CB-17/SCID mice into which cPDLSCs had been transplanted. These results suggest that cPDLSCs are clonogenic, highly proliferative, and have multidifferentiation potential, and that they could be used as a new cellular therapeutic approach to facilitate successful and more predictable regeneration of periodontal tissue using a canine model of periodontal disease.