A dominant lethal genetic system for autocidal control of the Mediterranean fruitfly
A dominant lethal genetic system for autocidal control of the Mediterranean fruitfly
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DOI：
10.1038/nbt1071
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发表时间：
2005-04-01
影响因子：
46.9
通讯作者：
Alphey, L
中科院分区：
文献类型：
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作者：
Gong, P;Epton, MJ;Alphey, L
用来控制害虫的不育昆虫技术(SIT)依赖于释放大量辐射不育的昆虫。辐射会对释放的昆虫的后续表现产生负面影响(1-4)，从而对控制计划的成本和效果产生负面影响(5)。这一问题以及与当前SIT计划相关的其他问题可以通过使用重组DNA方法和分子遗传学来克服(6-12)。在这里，我们描述了地中海果蝇品系的构建，该品系含有四环素可抑制反式激活因子(TTA)，这种反式激活因子在杂合子后代的早期发育阶段会导致死亡，但对亲本转基因TTA昆虫的生存几乎没有影响。我们表明，这些性质应该被证明有利于害虫控制计划的实施。
The Sterile Insect Technique (SIT) used to control insect pests relies on the release of large numbers of radiation-sterilized insects. Irradiation can have a negative impact on the subsequent performance of the released insects(1-4) and therefore on the cost and effectiveness of a control program(5). This and other problems associated with current SIT programs could be overcome by the use of recombinant DNA methods and molecular genetics(6-12). Here we describe the construction of strains of the Mediterranean fruit fly (medfly) harboring a tetracycline-repressible transactivator (tTA) that causes lethality in early developmental stages of the heterozygous progeny but has little effect on the survival of the parental transgenic tTA insects. We show that these properties should prove advantageous for the implementation of insect pest control programs.