Functional redundancy of the Notch gene family during mouse embryogenesis:: Analysis of Notch gene expression in Notch3-deficient mice
Functional redundancy of the Notch gene family during mouse embryogenesis:: Analysis of Notch gene expression in Notch3-deficient mice
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DOI：
10.1016/j.bbrc.2005.03.241
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发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Hanaoka, K
中科院分区：
文献类型：
--
作者：
Kitamoto, T;Takahashi, K;Hanaoka, K
Notch 3基因是Notch基因家族的成员，在发育过程中在多种组织中表达。我们产生并分析了Notch 3缺陷小鼠，以评估Notch 3基因的体内作用。与Krebs等人[Characterization of Notch 3-deficient mice：normal embryonic development and absence of genetic interactions with a Notch 1 mutation，Genesis 37（3）（2003）139-143]的先前观察一致，尽管Notch 3在各种胚胎组织中大量表达，但Notch 3（-/-）小鼠是存活的、可育的并且正常发育。我们研究了Notch 1，2和4表达的细节Notch 3（-/-）胚胎与野生型胚胎相比。结果，我们发现Notch 3的缺陷不影响Notch 1，2的表达。和4，并且Notch 1或Notch 2，或有时两者，总是在所有检测的Notch 3表达组织中表达。这些结果支持了其他Notch基因在胚胎发育过程中功能上补偿Notch 3的想法。我们还调查了Notch 3（-/-）小鼠的成年组织，发现10周龄小鼠的胸腺细胞明显较少。因此，胸腺可能是Notch 3缺陷影响的靶组织。(c)2005年爱思唯尔公司All rights reserved.
The Notch3 gene, a member of the Notch gene family, is expressed in a wide variety of tissues during development. We generated and analyzed Notch3-deficient mice to assess the in vivo role of the Notch3 gene. Consistent with previous observation of Krebs et al. [Characterization of Notch3-deficient mice: normal embryonic development and absence of genetic interactions with a Notch1 mutation, Genesis 37 (3) (2003) 139-143], the Notch3(-/-) mice were viable, fertile, and developed normally despite abundant expression of Notch3 in various embryonic tissues. We examined the details of Notch1, 2, and 4 expressions in the Notch3(-/-) embryos compared with those in wild-type etubryos. As a result, we found that a deficiency in Notch3 did not affect the expression of Notch1, 2. and 4, and that either Notch1 or Notch2, or sometimes both, was always expressed in all Notch3-expressing tissues examined. These results support the idea that other Notch genes functionally compensate for Notch3 during embryonic development. We also surveyed the adult tissues of Notch3(-/-) mice and found significantly fewer thymocytes in 10-week-old mice. Therefore, the thymus might be a target tissue affected by Notch3 deficiency. (c) 2005 Elsevier Inc. All rights reserved.