A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka
A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka
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DOI：
10.1371/journal.pbio.3000185
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发表时间：
2019-04-01
期刊：
影响因子：
9.8
通讯作者：
Schartl, Manfred
中科院分区：
文献类型：
--
作者：
Herpin, Amaury;Schmidt, Cornelia;Schartl, Manfred
DMRT1是一种高度保守的转录因子，参与脊椎动物性腺发育的调控。在青竹中，作为性别决定基因的dmrt1的副本具有严格的时间和空间控制的性腺表达模式。除了转录调控外，3‘非编码区(D3U-box)中的一个序列基序还介导了青竹和其他脊椎动物dmrt1 mRNAs的转录稳定性。我们在这里表明，在青竹中，两种具有拮抗特性的RNA结合蛋白针对这个D3U-box，促进生殖细胞中的RNA稳定或胞体中的降解。D3U-box在其他生殖细胞转录本中也是保守的，使它们对相同的RNA结合蛋白产生反应。后生动物dmrt1基因中D3U-box基序的进化保守，以及生殖细胞亚群中靶向RNA结合蛋白的保留表达模式，表明这种控制RNA稳定性的新机制不仅适用于鱼类，还可能适用于其他脊椎动物。
Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1-acting as master sex-determining gene-has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3' UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans-together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells-suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.