VITAMIN-E, LDL, AND ENDOTHELIUM - BRIEF ORAL VITAMIN SUPPLEMENTATION PREVENTS OXIDIZED LDL-MEDIATED VASCULAR INJURY IN-VITRO
VITAMIN-E, LDL, AND ENDOTHELIUM - BRIEF ORAL VITAMIN SUPPLEMENTATION PREVENTS OXIDIZED LDL-MEDIATED VASCULAR INJURY IN-VITRO
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DOI：
10.1161/01.atv.13.12.1779
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发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
VERCELLOTTI, GM
中科院分区：
文献类型：
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作者：
BELCHER, JD;BALLA, J;VERCELLOTTI, GM
In previously reported in vitro studies, we found that heme, a physiologically widespread hydrophobic iron compound, can rapidly generate oxidized low-density lipoprotein (LDL), which then becomes cytotoxic to cultured vascular endothelial cells; both LDL oxidation and endothelial cytotoxicity were inhibited by incubation with exogenous alpha-tocopherol (vitamin E) or ascorbic acid (vitamin C). Seeking relevance to in vivo conditions, we performed a study in which 10 human volunteers were given daily antioxidant supplements of 800 IU of DL-alpha-tocopherol acetate alone or in combination with 1000 mg of ascorbic acid for 2 weeks. LDL resistance to heme oxidation ex vivo, as measured by the lag time for conjugated-diene formation, increased by as much as threefold from a mean+/-SD of 58+/-11 to 104+/-18 minutes (P
