Functional characterization of the regulators of calcineurin in Candida glabrata
Functional characterization of the regulators of calcineurin in Candida glabrata
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DOI：
10.1111/j.1567-1364.2011.00751.x
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发表时间：
2011-12-01
影响因子：
3.2
通讯作者：
Kohno, Shigeru
中科院分区：
文献类型：
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作者：
Miyazaki, Taiga;Izumikawa, Koichi;Kohno, Shigeru
丝氨酸-苏氨酸特异性蛋白磷酸酶钙调神经磷酸酶是真菌中各种应激反应的关键介质。在此，我们表征了致病真菌光滑念珠菌中钙调神经磷酸酶（RCN）内源调节因子 Rcn1 和 Rcn2 的功能。 Rcn1 对钙调磷酸酶信号传导具有抑制和刺激作用，但 Rcn2 仅表现出抑制活性。对缺乏 RCN、钙调磷酸酶或两者的光滑念珠菌菌株的表型分析表明，钙调神经磷酸酶需要 Rcn1，而不是 Rcn2，才能实现光滑念珠菌的抗真菌耐受性。
The serine-threonine-specific protein phosphatase calcineurin is a key mediator of various stress responses in fungi. Herein, we characterized functions of the endogenous regulators of calcineurin (RCNs), Rcn1 and Rcn2, in the pathogenic fungus Candida glabrata. Rcn1 exerted both inhibitory and stimulatory effects on calcineurin signaling, but Rcn2 displayed only inhibitory activity. Phenotypic analyses of C. glabrata strains lacking either RCNs, calcineurin, or both revealed that calcineurin requires Rcn1, but not Rcn2, for antifungal tolerance in C. glabrata.