Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice.
Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice.
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黑茶花青素对神经干细胞和衰老小鼠的抗衰老作用
DOI：
10.18632/aging.103955
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发表时间：
2020-09-12
期刊：
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Gao J;Wu Y;He D;Zhu X;Li H;Liu H;Liu H
衰老与神经功能障碍和认知能力下降有关。类黄酮在小鼠模型的抗衰老研究中非常有前景。茶藨花青素富含丰富的黄酮类化合物，但其抗衰老生物活性尚不清楚。在本研究中，我们制备了红花花青素提取物，并在体内和体外分析了其对神经干细胞（NSC）衰老的影响。我们分离了小鼠 NSC，并使用细胞计数试剂盒 8 (CCK-8)、细胞周期、活性氧 (ROS) 和免疫荧光方法来分析 R. meyeri 花青素以及柚皮素 (Nar) 的抗衰老作用，代谢分析表明柚皮素 (Nar) 是 R. meyeri 花青素中重要的黄酮类化合物。 RNA测序（RNA-seq）和酶联免疫吸附测定（ELISA）方法也被用来研究Nar特异性的抗衰老机制。 R. meyeri花青素处理后，NSC增殖加速，NSC衰老标志物减少，P16ink4a表达减少。 R. meyeri 花青素治疗还在体内和体外逆转了年龄依赖性神经元损失。 Nar 在体外阻断 mNSC 衰老，并通过下调血浆 TNF-α 蛋白来改善衰老小鼠的空间记忆和认知能力。这些发现表明，R. meyeri 花青素通过 Nar 诱导的体内 TNF-α 蛋白水平降低，增加 NSC 增殖并改善衰老过程中的神经发生。
Aging is associated with neurological impairment and cognitive decline. Flavonoids are very promising in anti-aging research in mouse models. Ribes meyeri anthocyanins are rich in abundant flavonoids, but their anti-aging biological activities remain unknown. In this study, we prepared an R. meyeri anthocyanin extract and analyzed its effects on neural stem cell (NSC) senescence in vivo and in vitro. We isolated mouse NSCs and used cell counting kit-8 (CCK-8), cell cycle, reactive oxygen species (ROS), and immunofluorescence methods to analyze the anti-aging effects of R. meyeri anthocyanins as well as naringenin (Nar), which metabolic analysis revealed as an important flavonoid in R. meyeri anthocyanins. RNA-sequencing (RNA-seq) and enzyme-linked immuno sorbent assay (ELISA) methods were also used to investigate Nar-specific mechanisms of anti-aging. After R. meyeri anthocyanin treatment, NSC proliferation accelerated, and NSCs had decreased senescence markers, and reduced P16ink4a expression. R. meyeri anthocyanin treatment also reversed age-dependent neuronal loss in vivo and in vitro. Nar blocked mNSC aging in vitro and improved spatial memory and cognitive abilities in aging mice through downregulation of plasma TNF-α protein. These findings suggest that R. meyeri anthocyanins increase NSC proliferation and improve neurogenesis with aging via Nar-induced reductions in TNF-α protein levels in vivo.
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影响因子：
7.9
作者：
Park, Sunwoo;Lim, Whasun;Song, Gwonhwa
通讯作者：
Song, Gwonhwa
单细胞转录组学揭示了不同神经干/祖细胞亚群中与衰老相关的基因特征和改变
影响因子：
21.1
作者：
Shi Z;Geng Y;Liu J;Zhang H;Zhou L;Lin Q;Yu J;Zhang K;Liu J;Gao X;Zhang C;Yao Y;Zhang C;Sun YE
通讯作者：
Sun YE
影响因子：
5.6
作者：
Bullone M;Lavoie JP
通讯作者：
Lavoie JP
影响因子：
5.3
作者：
Maslov, AY;Barone, TA;Pruitt, SC
通讯作者：
Pruitt, SC
较高的饮食花青素和黄酮醇的摄入量与美国成年人的抗炎作用有关。
DOI：
10.3945/ajcn.115.108555
发表时间：
2015-07
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Cassidy A;Rogers G;Peterson JJ;Dwyer JT;Lin H;Jacques PF
通讯作者：
Jacques PF