In vitro characteristics of human glioblastomas maintained in organ culture systems. Light microscopy observations.
In vitro characteristics of human glioblastomas maintained in organ culture systems. Light microscopy observations.
复制标题
器官培养系统中维持的人胶质母细胞瘤的体外特征。
DOI：
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发表时间：
1973
影响因子：
6
通讯作者：
V. L. Foley
中科院分区：
文献类型：
--
作者：
L. J. Rubinstein;M. Herman;V. L. Foley
使用三维海绵泡沫基质技术或Millipore过滤平台技术，描述了在器官培养系统中生长的五种人类胶质母细胞瘤的光学显微镜观察结果。许多培养成功维持2个月以上；其中一组维持了137天。胶质瘤细胞对海绵泡沫基质的侵袭是不可避免的。在离体后122天可观察到有丝分裂象。所有肿瘤均出现分化良好的星形胶质细胞；在培养的后期，他们经常表现出胶质纤维增生。少突胶质细胞也见于一例。体内未见假性化和内皮细胞增生的特征。相反，一些外植体的血管间质表现出明显的硬化倾向，血管腔增厚、透明化和逐渐闭塞。用该技术培养的外植体的培养行为与在胶原包膜盖上培养的姐妹培养体的培养行为进行了比较。器官培养技术显示出一些重要的生长特性差异。与胶原包膜盖层培养相反，成纤维细胞增殖受到抑制，在包膜盖层上4至6周后有时会出现的明显的间变性细胞特征在器官培养中没有出现。
Light microscopy observations are described on five human glioblastomas grown in organ culture systems, using either a three-dimensional sponge foam matrix technic or a Millipore filter platform technic. Many cultures were successfully maintained for more than 2 months; one was maintained for 137 days. Invasion of the sponge foam matrix by glioma cells was invariably demonstrated. Mitotic figures were seen up to 122 days after explantation. All tumors showed the development of well-differentiated astrocytes; they often showed increased glial fibrillogenesis in the later stages of culture. Oligodendroglia were also demonstrated in one case. The in vivo features of pseudopalisading and endothelial proliferation were not seen. On the contrary, the vascular stroma of several explants showed a marked tendency to undergo sclerosis, with thickening, hyalinization and gradual obliteration of the vascular lumens. The cultural behavior of the explants with this technic was compared with that of sister cultures grown on collagen-coated coverslips. The organ culture technics revealed some important differences in growth characteristics. In contrast to what was seen in collagen-coated coverslip cultures, fibroblastic proliferation was inhibited, and the marked anaplastic cellular features that were sometimes manifest after 4 to 6 weeks on coated coverslips were not seen in the organ cultures.