Mouse mammary carcinoma cell line (BALB/c-MC) stimulates osteoclast formation from mouse bone marrow cells through cell-to-cell contact
Mouse mammary carcinoma cell line (BALB/c-MC) stimulates osteoclast formation from mouse bone marrow cells through cell-to-cell contact
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DOI：
10.1016/s8756-3282(98)00065-9
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发表时间：
1998-07-01
期刊：
影响因子：
4.1
通讯作者：
Nagata, N
中科院分区：
文献类型：
--
作者：
Ono, K;Akatsu, T;Nagata, N
我们最近报道了在一些小鼠肿瘤细胞系和骨髓细胞的共培养中形成了大量的破骨细胞（OC）。在本研究中，我们研究了BALB/c-MC细胞诱导OC的机制，BALB/c-MC在共培养中剂量依赖性地促进OC的形成。在共培养中，BALB/c-MC与骨髓细胞之间的直接细胞-细胞接触被膜阻断剂抑制，OC的形成不受刺激。加入10（-7）-10（-6）mol/L吲哚美辛可完全阻断共培养对OC形成的刺激作用。细胞间接触共培养的培养基中前列腺素E-2（PGE（2））的浓度高于无细胞间接触共培养或单独骨髓培养的培养基中前列腺素E-2（PGE（2））的浓度，并且达到足以诱导OC的水平（11.9 +/- 5.3 ng/mL [约3.4 x 10（-8）mol/L]），当BALB/ c-MC或骨髓细胞用福尔马林固定，然后分别与骨髓细胞或BALB/c-MC共培养时，固定BALB/c-MC与骨髓细胞共培养时，培养液中的β-CD含量增加，而固定BALB/c-MC与骨髓细胞共培养时，培养液中的β-CD含量没有增加。这些结果表明，BALB/c-MC通过直接细胞与细胞接触刺激OC形成，骨髓细胞和通过直接细胞与细胞接触从骨髓细胞释放的PGE参与细胞系的OC形成。(Bone 23：27-32; 1998）（C）1998由Elsevier Science Inc. All rights reserved.
We recently reported that numerous osteoclasts (OC) were formed in cocultures of some mouse cancer cell lines and bone marrow cells. In this study, we examined mechanisms by which one of the cell lines, BALB/c-MC, induces OC, BALB/c-MC dose dependently stimulated OC formation in cocultures, In cocultures where direct cell-to-fell contact between BALB/c-MC and bone marrow cells was inhibited by membrane biters, OC formation was not stimulated. The stimulation of OC formation in the coculture was completely abolished by adding 10(-7)-10(-6) mol/L indomethacin.,The concentration of prostaglandin E-2 (PGE(2)) in the culture media of cocultures with cell-to-cell contact was higher than that of cocultures without cell-to-cell contact or marrow cultures alone, and it reached levels sufficient to induce OC (11.9 +/- 5.3 ng/mL [about 3.4 x 10(-8) mol/L]), When BALB/ c-MC or bone marrow cells were fixed with formalin and then cocultured with bone marrow cells or BALB/c-MC, respectively, the concentration of PGE, in the culture media of cocultures of fixed BALB/c-MC and bone marrow cells increased, whereas that of cocultures of BALB/c-MC and fixed bone marrow cells did not increase. These results indicate that BALB/c-MC stimulate OC formation through direct cell-to-cell contact,vith bone marrow cells, and PGE, released from bone marrow cells through direct cell-to-cell contact are involved in OC formation by the cell line. (Bone 23:27-32; 1998) (C) 1998 by Elsevier Science Inc. All rights reserved.